Distinct genetic diversity of carbapenem-resistant acinetobacter baumannii from Colombian hospitals

Miniatura

Archivos

Correa, Adriana_2018.pdf (362.36 KB)

Fecha

2018

Autores

Título de la revista

Publicado en

Microbial Drug Resistance, 1076-6294, Vol 24, Num 1, 2018, pag 48-54

Publicado por

Mary Ann Liebert

URI

https://hdl.handle.net/20.500.12495/2893

URL de la fuente

https://www.liebertpub.com/doi/abs/10.1089/mdr.2016.0190?rfr_dat=cr_pub%3Dpubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&journalCode=mdr

Enlace a contenidos multimedia

ISSN de la revista

Título del volumen

Resumen

Descripción

Abstract

The global success of multidrug-resistant Acinetobacter baumannii has been associated with the dissemination of a high-risk clone designated clonal complex (CC) 92B (Bartual scheme)/CC2P (Pasteur scheme), which is the most frequent genetic lineage in European, Asian, and North American carbapenem-resistant Acinetobacter isolates. In these isolates, carbapenem resistance is mainly mediated by β-lactamases encoded by blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, and/or blaOXA-58-like genes. In this study, we characterized the population genetics of 121 carbapenem-resistant A. baumannii complex isolates recovered from 14 hospitals in seven cities in Colombia (2008–2010). Multiplex PCR was used to detect blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, and blaOXA-58-like genes. Molecular typing was performed using pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). PCR showed that 118 (97.5%) of the isolates were positive for both blaOXA-23-like and blaOXA-51-like genes, and three other isolates were only positive for blaOXA-51-like. PFGE identified 18 different pulsotypes, while MLST identified 11 different sequence types (STs), seven of which had not been previously described in Acinetobacter. None of the STs found in this study was associated with CC92B/CC2P. The most widespread STs in our isolates belonged to ST636 and their single-locus variants ST121/ST124/ST634 (CC636B) followed by STs belonging to CC110B. Our observations suggest a wide distribution of diverse A. baumannii complex clones containing blaOXA-23-like in Colombian hospitals (especially CC636B and CC110B) that differ from the high-risk clones commonly found in other regions of the world, indicating a distinct molecular epidemiology of carbapenem-resistant Acinetobacter spp. in Colombia.

Palabras clave

Keywords

Acinetobacter baumannii, Antibiotic resistance, Carbapenemase, OXA enzymes, Multilocus sequence typing

Temáticas

Resistencia a medicamentos
Bacilos y cocos aerobios gramnegativos
Técnicas de tipificación bacteriana

Citación

Colecciones

Artículos