Diminuição da infecção por rotavírus em células MA104 pela união de proteínas de extratos probióticos a receptores integrina β3 e Hsc70

Miniatura

Archivos

Salas-Cárdenas, Sandra Patricia.pdf (754.25 KB)

Fecha

2018

Autores

Título de la revista

Publicado en

Universitas Scientiarum, 2027-1352, Vol 23, Num 2, 2018, pag 219-239

Publicado por

Pontificia Universidad Javeriana

URI

https://hdl.handle.net/20.500.12495/2871

URL de la fuente

https://revistas.javeriana.edu.co/index.php/scientarium/article/view/23341

Enlace a contenidos multimedia

ISSN de la revista

Título del volumen

Resumen

Descripción

Abstract

Probiotic bacteria are microorganisms beneficial to human health, useful to improving biological conditions. Thanks to probiotic bacteria the symptoms of viral infections can be alleviated. Different mechanisms whereby probiotic bacteria exert they antiviral effect have been proposed. The aim of this study was to determine whether probiotic bacteria extracts bind to receptors of host cells susceptible of rotavirus (RV) infection. To accomplish this objective, four probiotic bacterial strains of Lactobacillus spp. and Bifidobacterium spp. were tested. Probiotic extracts were obtained after bacterial growth, cell lysis and centrifugation. Obtained probiotic extracts were used in assays to interfere with adhesion and penetration of a RV strain in the mammal cell line MA104. Furthermore, the interaction between probiotic extracts and MA104 cell receptors was evaluated by co-immunoprecipitation assays using anti-β3-integrins and anti-Hsc70 antibodies. All four probiotic, protein-rich, extracts reduced RV infections in MA104 cells, suggesting a successful antiviral activity mediated by these probiotic extracts. All probiotic extracts significantly exerted their antiviral activity by interfering with RV adhesion on MA104 cell receptors, with proteins in probiotic extracts competitively interacting with cell surface receptors necessary to RV infection. Co-immunoprecipitation assay results showed that proteins in probiotic extracts were able to bind to β3-integrinsand Hsc70, which are two cellular receptors required to viral infection. The most significant contribution of this study is an insight into the mechanisms of probiotic antiviral activity, thus expanding current probiotics fundamental knowledge

Palabras clave

Keywords

Probiotics, Antiviral effect, Rotavirus, Hsc70, Integrin ß3

Temáticas

Probióticos
Bacterias
Reoviridae

Citación

Colecciones

Artículos