Levels of soluble ST2 in serum associated with severity of dengue due to tumour necrosis factor alpha stimulation

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dc.contributor.authorHoughton-Triviño, Natalia
dc.contributor.authorSalgado, Doris M.
dc.contributor.authorRodríguez, Jairo A.
dc.contributor.authorBosch, Irene
dc.contributor.authorCastellanos, Jaime
dc.contributor.orcidCastellanos, Jaime [0000-0003-1596-8383]
dc.date.accessioned2021-02-12T14:38:55Z
dc.date.available2021-02-12T14:38:55Z
dc.date.issued2010
dc.description.abstractenglishThe interleukin-1 receptor-like-1 protein (IL1RL1), also known as ST2, has been shown previously to regulate T-cell function and is produced by T cells and endothelial cells. It was reported recently to be elevated in mild dengue patients during acute disease. The ST2 gene encodes several splice products: L (long), V (short) and s (soluble). A cohort of 38 patients with dengue haemorrhagic fever (DHF) and mild dengue fever (DF) were evaluated using a secreted soluble ST2 (sST2) ELISA. The RNA expression of ST2 was evaluated by real-time quantitative RT-PCR using patients' peripheral blood mononuclear cells (PBMCs) and in vitro using human umbilical vein endothelial cells (HUVECs) exposed to sera from dengue patients. DHF patients had higher levels of serum sST2, tumour necrosis factor alpha (TNF-α), interleukin (IL)-8 and IL-10 compared with DF patients and normal healthy control individuals. However, viraemia was indistinguishable between mild and severe cases. No changes in ST2 mRNA expression were found in PBMCs from these two groups of dengue patients. In vitro, sST2 was elevated in HUVECs treated with patient sera. Neutralization of TNF-α in patient sera by pre-treatment with a TNF-α antibody inhibited the upregulation of sST2 expression in HUVECs. These results implicate serum TNF-α in the modulation of expression of sST2 in an in vitro system, and indicate that sST2 could be associated with the severity of disease. Further studies to determine whether sST2 levels are predictive of the severe form of the disease and the role of sST2 in immune regulation are warranted.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1099/vir.0.012971-0
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1465-2099
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/5302
dc.language.isoeng
dc.publisherMicrobiology Societyspa
dc.publisher.journalJournal of General Virologyspa
dc.relation.ispartofseriesJournal of General Virology, 1465-2099, Vol. 91, No. 3, 2010, p. 697-706spa
dc.relation.urihttps://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.012971-0#tab2
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2010
dc.rights.localAcceso abiertospa
dc.subject.keywordsBiomarkers
dc.subject.keywordsCell line
dc.subject.keywordsGene expression profiling
dc.titleLevels of soluble ST2 in serum associated with severity of dengue due to tumour necrosis factor alpha stimulationspa
dc.title.translatedLevels of soluble ST2 in serum associated with severity of dengue due to tumour necrosis factor alpha stimulationspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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