Quantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatography

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dc.contributor.authorWhitted, Crystal L.
dc.contributor.authorRodríguez Aguirre, Oscar Eduardo
dc.contributor.authorPalau, Victoria
dc.contributor.authorTorrenegra G., Ruben Dario
dc.contributor.authorHarirforoosh, Sam
dc.contributor.orcidRodríguez Aguirre, Oscar Eduardo [0000-0002-5934-0451]
dc.date.accessioned2019-07-30T20:50:36Z
dc.date.available2019-07-30T20:50:36Z
dc.date.issued2017
dc.description.abstractenglishBackground Antineoplastic activity has been previously shown for two isomeric flavones, 5,7-dihydroxy-3,6,8-trimethoxy flavone (flavone A) and 3,5-dihydroxy-6,7,8-trimethoxy flavone (flavone B), against colon cancer cell lines (Thomas et al. in PLoS ONE 7:e39806, 5). Here, we present modified methods for the extraction and quantification of flavones A and B in rat colon tissue after intravenous dosing via high performance liquid chromatography, from the originally described procedure for extraction and quantification in rat plasma (Whitted et al. in J Chromatogr B Analyt Technol Biomed Life Sci 1001:150–155, 7). Results Modifications included tissue homogenization (1 g tissue: 2 mL water), filtration of the supernatant with a PVDF membrane, and the use of only one calibration curve to determine the concentration of each flavone in colon tissue. Good separation was achieved and representative equations were linear with r 2 ≥ 0.99 for both flavones. Precision and accuracy for flavone A ranged from 0.88–24.03 and 109–116%. Precision and accuracy for flavone B ranged from 1.62–33.56 and 98–113%. Concentrations of 1639 ± 601 ng/g flavone A and 5975 ± 2480 ng/g of flavone B were detected in rat colon tissue 6 h post dosing. Conclusions Modifications to the extraction methods for flavone A and flavone B from rat colon tissue had good separation, precision, and accuracy.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/s13104-016-2358-y
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1756-0500
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/1575
dc.language.isoeng
dc.publisherBiomed Centralspa
dc.publisher.journalBMC Research Notesspa
dc.relation.ispartofseriesBMC Research Notes, 1756-0500, Vol. 10,Nro.29, 2017, p.1-6spa
dc.relation.urihttps://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-016-2358-y
dc.rightsAttribution 4.0 International*
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf447
dc.rights.creativecommons2017
dc.rights.localAcceso abiertospa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.decsNeoplasiasspa
dc.subject.decsCompuestos heterocíclicosspa
dc.subject.decsFlavanonasspa
dc.subject.keywords5,7-Dihydroxy-3,6,8-trimethoxy flavonespa
dc.subject.keywords3,5-Dihydroxy-6,7,8-trimethoxy flavonespa
dc.subject.keywordsColonspa
dc.subject.keywordsFlavonoidsspa
dc.titleQuantification of two isomeric flavones in rat colon tissue using reverse phase high performance liquid chromatographyspa
dc.typearticlespa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localartículospa

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