Molecular characterisation of carbapenem-resistant Enterobacter cloacae complex in Colombia: blaKPC and the 'changing landscape

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dc.contributor.authorde la Cadena, Elsa
dc.contributor.authorCorrea, Adriana
dc.contributor.authorMuñoz, Juan Sebastián
dc.contributor.authorRojas, Laura J.
dc.contributor.authorHernández-Gómez, Cristhian
dc.contributor.authorPallares, Christian José
dc.contributor.authorPerez, Federico
dc.contributor.authorBonomo, Robert A.
dc.contributor.authorVillegas, María Virginia
dc.contributor.orcidde la Cadena, Elsa [0000-0003-0361-7893]
dc.contributor.orcidPallares, Christian José [0000-0002-6093-7845]
dc.contributor.orcidVillegas, María Virginia [0000-0003-1898-9067]
dc.date.accessioned2020-05-18T09:59:22Z
dc.date.available2020-05-18T09:59:22Z
dc.date.issued2018
dc.description.abstractenglishObjectives The aim of this study was to examine the population structure of representative carbapenem-resistant Enterobacter cloacae complex (CR-Ecl) isolates from eight different Colombian regions and to characterise their associated β-lactamases. Methods A total of 28 CR-Ecl isolates collected in Colombia between 2009–2013 through the Colombian Nosocomial Network were included in this study. Antimicrobial susceptibility testing was performed by the broth microdilution method. Molecular detection of carbapenemase and extended-spectrum β-lactamase (ESBL) genes and the presence of transposon Tn4401 was evaluated by PCR and DNA sequencing. Genetic relatedness was assessed by multilocus sequencing typing (MLST) and repetitive sequence-based PCR (rep-PCR). Results PCR and DNA sequencing revealed that 19/28 (68%) of the CR-Ecl isolates carried blaKPC-2. Analysis of the genetic environment found blaKPC-2 within transposon Tn4401b in 8/19 isolates (42%). Population genetic analysis using rep-PCR revealed four clonal groups. MLST showed a variety of sequence types (STs), among which ST510 was the most common (10/28 isolates; 36%). Conclusions blaKPC-2 was discovered as the most common mechanism of carbapenem resistance in CR-Ecl and was disseminated among different STs. Although none of the previously reported major clonal complexes were identified, it appears that local strain lineages are associated with the spread of blaKPC within CR-Ecl in various regions of Colombia.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.jgar.2017.12.008
dc.identifier.issn2213-7173
dc.identifier.urihttps://hdl.handle.net/20.500.12495/2931
dc.language.isoeng
dc.publisher.journalJournal of Global Antimicrobial Resistancespa
dc.relation.ispartofseriesJournal of Global Antimicrobial Resistance, 2213-7173, Vol 13, 2018, pag 184-189spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S2213716517302382?via%3Dihub
dc.rights.creativecommons2018
dc.rights.localAcceso cerradospa
dc.subject.decsEnterobacteriaceaespa
dc.subject.decsTipificación de secuencias multilocusspa
dc.subject.decsTipificación molecularspa
dc.subject.keywordsEnterobacter cloacaespa
dc.subject.keywordsCarbapenem resistancespa
dc.subject.keywordsMultilocus sequence typingspa
dc.titleMolecular characterisation of carbapenem-resistant Enterobacter cloacae complex in Colombia: blaKPC and the 'changing landscapespa
dc.title.translatedMolecular characterisation of carbapenem-resistant Enterobacter cloacae complex in Colombia: blaKPC and the 'changing landscapespa
dc.typearticlespa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localartículospa

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