Strain-specific adaptations of streptococcus mitis-oralis to serial in vitro passage in daptomycin (DAP): genotypic and phenotypic characteristics

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dc.contributor.authorMishra, Nagendra Nath
dc.contributor.authorTran, Truc T.
dc.contributor.authorArias, César A.
dc.contributor.authorSeepersaud, Ravin
dc.contributor.authorSullam, Paul M.
dc.contributor.authorBayer, Arnold S.
dc.date.accessioned2020-09-02T00:08:21Z
dc.date.available2020-09-02T00:08:21Z
dc.description.abstractenglishAbstract:Viridans group streptococci (VGS), especially theStreptococcus mitis-oralissubgroup,are pivotal pathogens in a variety of invasive endovascular infections, including “toxic shock”in neutropenic cancer patients and infective endocarditis (IE). Previously, we showed that theserialin vitropassage ofS. mitis-oralisstrains in sublethal daptomycin (DAP) resulted in rapid,high-level and stable DAP-resistance (DAP-R), which is accompanied by distinct changes in severalgenotypic and phenotypic signatures: (1) the disappearance of two key membrane phospholipids,phosphatidylglycerol (PG) and cardiolipin (CL); (2) increased membrane fluidity; (3) increased positivesurface charge; (4) single nucleotide polymorphisms (SNPs) in two loci involved in CL biosynthesis(pgsA; cdsA); and (5) DAP hyperaccumulation. The current study examined these same metricsfollowingin vitroserial DAP passages of a separate well-characterizedS. mitis-oralisbloodstreamisolate (SF100). Although some metrics seen in prior DAP post-passage strains were recapitulated withSF100 (e.g.,pgsASNPs, enhanced membrane fluidity), we observed the following major differences(comparing the parental versus post-passage variant): (1) no change in PG content; (2) reduced,but notabsent, CL, with enhancement in phosphatidic acid (PA) content; (3) an unusual pattern of CLlocalization; (4) significantly decreased positive surface charge; (5) no difference in DAP accumulation;and (6) nocdsASNPs. Thus,S. mitis-oralisstrains are not “pre-programmed” phenotypically and/orgenotypically to adapt in an identical manner during the evolution of the DAP-R.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://www.x-mol.com/paperRedirect/1294694111828910080
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn2079-6382
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlhttps://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/3915
dc.language.isoeng
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)spa
dc.publisher.journalAntibioticsspa
dc.relation.ispartofseriesAntibiotics, 2079-6382, Vol. 9, Nro. 8, 2020. p. 1-10spa
dc.relation.urimdpi.com/2079-6382/9/8/520
dc.rightsAttribution 4.0 International*
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2020-08-15
dc.rights.localAcceso abiertospa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.keywordsDaptomycin resistancespa
dc.subject.keywordsStreptococcus mitis-oralisspa
dc.titleStrain-specific adaptations of streptococcus mitis-oralis to serial in vitro passage in daptomycin (DAP): genotypic and phenotypic characteristicsspa
dc.title.translatedStrain-specific adaptations of streptococcus mitis-oralis to serial in vitro passage in daptomycin (DAP): genotypic and phenotypic characteristicsspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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