Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells
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Journal of supercritical fluids, 0896-8446, Vol. 15, 2019
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Elsevier
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Abstract
Mango seed kernel(MSK) has demonstrated to be a valuable source of bioactive phenolic compounds with
antiproliferative activity against colon cancer cell lines. In this work, a phenolic MSK extract obtained
by sequential pressurized liquid extraction (PLE) was submitted to supercritical antisolvent fractionation (SAF) to attain enriched fractions with improved inhibitory effect on HT-29 colon cancer cells.
SAF was optimized using a Box-Behnken experimental design and considering as main factors: pressure, percentage of water in the feed extract and feed/sc−CO2 flow rate ratio. The highest inhibitory cell
proliferation effect (70.51 ± 1.14%) was obtained in the extract using 50% of water v/v in the feed and
0.0625 feed/sc−CO2 ratio at 15 MPa. A targeted phytochemical profiling by LC-q-TOF-MS/MS followed
by a multivariate statistical analysis of the observed bioactivity and the chemical composition evidenced
the presence of characteristic compounds which might explain the enhanced antiproliferative activity of
the optimal SAF extract.