A Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strains

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dc.contributor.authorGutiérrez-Barbosa, Hernando
dc.contributor.authorMedina-Moreno, Sandra
dc.contributor.authorPerdomo-Celis, Federico
dc.contributor.authorDavis, Harry
dc.contributor.authorCoronel-Ruiz, Carolina
dc.contributor.authorZapata, Juan C.
dc.contributor.authorChua, Joel V.
dc.date.accessioned2023-07-19T21:09:50Z
dc.date.available2023-07-19T21:09:50Z
dc.date.issued2023
dc.description.abstractenglishHumanized mice are an invaluable tool for investigating human diseases such as cancer, infectious diseases, and graft-versus-host disease (GvHD). However, it is crucial to understand the strengths and limitations of humanized mice and select the most appropriate model. In this study, we describe the development of the human lymphoid and myeloid lineages using a flow cytometric analysis in four humanized mouse models derived from NOD mice xenotransplanted with CD34+ fetal cord blood from a single donor. Our results showed that all murine strains sustained human immune cells within a proinflammatory environment induced by GvHD. However, the Hu-SGM3 model consistently generated higher numbers of human T cells, monocytes, dendritic cells, mast cells, and megakaryocytes, and a low number of circulating platelets showing an activated profile when compared with the other murine strains. The hu-NOG-EXL model had a similar cell development profile but a higher number of circulating platelets with an inactivated state, and the hu-NSG and hu-NCG developed low frequencies of immune cells compared with the other models. Interestingly, only the hu-SGM3 and hu-EXL models developed mast cells. In conclusion, our findings highlight the importance of selecting the appropriate humanized mouse model for specific research questions, considering the strengths and limitations of each model and the immune cell populations of interest. © 2023 by the authors.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.3390/microorganisms11061548
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn2076-2607
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/11103
dc.language.isoeng
dc.publisherMultidisciplinary Digital Publishing Institutespa
dc.publisher.journalMicroorganismsspa
dc.relation.ispartofseriesMicroorganisms, 2076-2607, 11 (6), 2023, 1 - 19spa
dc.relation.urihttps://www.mdpi.com/2076-2607/11/6/1548
dc.rightsAtribución 4.0 Internacional*
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.localAcceso abiertospa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectHumanizaciónspa
dc.subjectModelo de ratón humanizadospa
dc.subjectXenoinjertospa
dc.subjectCD34spa
dc.subjectNSGspa
dc.subjectNCGspa
dc.subjectNOG-EXLspa
dc.subjectNSG-SGM3spa
dc.subjectLinfoidespa
dc.subjectMieloidespa
dc.subject.keywordsHumanizationspa
dc.subject.keywordsHumanized mouse modelspa
dc.subject.keywordsXenograftspa
dc.subject.keywordsCD34spa
dc.subject.keywordsNSGspa
dc.subject.keywordsNCGspa
dc.subject.keywordsNOG-EXLspa
dc.subject.keywordsNSG-SGM3spa
dc.subject.keywordsLymphoidspa
dc.subject.keywordsMyeloidspa
dc.titleA Comparison of Lymphoid and Myeloid Cells Derived from Human Hematopoietic Stem Cells Xenografted into NOD-Derived Mouse Strainsspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.coarversionhttps://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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