Emerging and existing mechanisms co-operate in generating diverse β-lactam resistance phenotypes in geographically dispersed and genetically disparate Pseudomonas aeruginosa strains

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dc.contributor.authorMartinez, Elena
dc.contributor.authorEscobar-Pérez, Javier
dc.contributor.authorMárquez, Carolina
dc.contributor.authorVilacoba, Elisabet
dc.contributor.authorCentrón, Daniela
dc.contributor.authorLeal, Aura L.
dc.contributor.authorSaavedra, Carlos
dc.contributor.authorSaavedra, Sandra Y.
dc.contributor.authorTovar, Catalina
dc.contributor.authorVanegas, Natasha
dc.contributor.authorStokes, H.W.
dc.contributor.orcidEscobar-Pérez, Javier [0000-0002-0432-6978]
dc.date.accessioned2021-02-02T14:12:55Z
dc.date.available2021-02-02T14:12:55Z
dc.date.issued2013
dc.description.abstractenglishβ-Lactam resistance in Pseudomonas aeruginosa clinical isolates is driven by a number of mechanisms. Whilst several are understood, how they act co-operatively in pathogenic strains is less clear. In some isolates, resistance profiles cannot always be explained by identifying the common resistance-determining pathways, suggesting that other mechanisms may be important. Pathogenic P. aeruginosa isolates from four countries were characterised by PCR. Quantitative expression analysis was also assessed for the activity of several pathways that influence antibiotic resistance, and culture experiments were conducted to test how random transposition of the insertion sequence IS26 during growth may influence resistance to some antibiotics. In most strains, antibiotic resistance was being driven by changes in multiple pathways and by the presence or absence of genes acquired by lateral gene transfer. Multiple mechanisms of resistance were prevalent in strains from all of the countries examined, although regional differences in the type of interacting mechanisms were apparent. Changes in chromosomal pathways included overexpression of AmpC and two efflux pumps. Also, gain or loss of IS26 at some chromosomal locations, most notably oprD, could influence resistance to carbapenems. IS26-related resistance was found in strains from Argentina and geographically linked Uruguay, but not in strains from either Colombia or Australia. Pseudomonas aeruginosa pathogenic strains are evolving to become multidrug-resistant in more complex ways. This is being influenced by single strains acquiring changes in numerous known pathways as well as by newly emerging resistance mechanisms in this species.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.jgar.2013.03.013
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn2213-7165
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlhttps://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/5234
dc.language.isoeng
dc.publisherElsevierspa
dc.publisher.journalJournal of Global Antimicrobial Resistancespa
dc.relation.ispartofseriesJournal of Global Antimicrobial Resistance, 2213-7165, Vol 1, Num 3, 2013, pag 135-142spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S2213716513000428?via%3Dihub
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2013-09
dc.rights.localAcceso abiertospa
dc.subject.keywordsPseudomonas aeruginosaspa
dc.subject.keywordsCarbapenemspa
dc.subject.keywordsEfflux pumpsspa
dc.subject.keywordsClass 1 integronspa
dc.subject.keywordsAntibiotic resistancespa
dc.subject.keywordsGenomic islandspa
dc.subject.keywordsIS26spa
dc.titleEmerging and existing mechanisms co-operate in generating diverse β-lactam resistance phenotypes in geographically dispersed and genetically disparate Pseudomonas aeruginosa strainsspa
dc.title.translatedEmerging and existing mechanisms co-operate in generating diverse β-lactam resistance phenotypes in geographically dispersed and genetically disparate Pseudomonas aeruginosa strainsspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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