NEUTRALIZACIÓN DE LOS VENENOS DE SERPIENTES CORALES Micrurus medemi, Micrurus sangilensis y Micrurus helleri POR EL ANTIVENENO ANTICORAL DEL INSTITUTO NACIONAL DE SALUD

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dc.contributor.advisorRodríguez Vargas, Ariadna
dc.contributor.advisorLorena Corredor, Zayda
dc.contributor.authorTriana Ceron, Miguel Angel
dc.contributor.orcidTriana Ceron, Miguel Angel [0000-0002-5347-9989]
dc.date.accessioned2024-08-08T16:56:41Z
dc.date.available2024-08-08T16:56:41Z
dc.date.issued2024-07
dc.description.abstractIntroducción: El accidente ofídico es la lesión subsecuente a la mordedura de serpiente, en la que se inoculan componentes lesivos diversos causantes de alteraciones fisiopatológicas que pueden llevar a la muerte del afectado. El tratamiento del envenenamiento implica la administración de antivenenos de origen equino, que proveen inmunorreactividad directa o cruzada frente al veneno de múltiples especies de serpientes pertenecientes al mismo género. La presencia de isoformas en las principales toxinas de interés médico junto a la baja inmunogenicidad de algunas proteínas da por consiguiente la neutralización parcial por parte de los antivenenos y una respuesta farmacológica subóptima. Actualmente se desconoce la respuesta de antivenenos de uso comercial frente a la mordedura de serpientes poco investigadas como Micrurus medemi, Micrurus sangilensis y Micrurus helleri, que son endémicas de Colombia, distribuidas en territorios con gran incidencia de accidentes ofídicos, como la región Andina, Orinoquía y Amazonía. Objetivo y metodología: El presente trabajo pretende evaluar la eficacia de inmunorreconocimiento del antiveneno anticoral polivalente del Instituto Nacional de Salud contra el veneno de estas tres especies, a través de cromatografía de afinidad, ensayos de inmunoadsorción ligada a enzimas y Western blot, con previa caracterización de los venenos por medio de electroforesis uni y bidimensional, así como cromatografía en fase líquida de alta resolución. Resultados: Los venenos de las tres Micrurus spp. presentaron variaciones interespecíficas en características fisicoquímicas, como peso molecular, punto isoeléctrico y grado de hidrofobicidad. Estas variaciones influyeron en la capacidad de inmunorreactividad del antiveneno anticoral polivalente producido por el Instituto Nacional de Salud (INS). Aunque el antiveneno presentó reactividad cruzada frente a los venenos de M. helleri, M. medemi y M. sangilensis, se observó un reconocimiento variable hacia las toxinas de bajo peso molecular. Esto demarca la importancia de realizar estudios adicionales en serpientes del género Micrurus en las que se desconoce su grado de reactividad, especialmente en regiones con alta incidencia de ofidiotoxicosis, con el fin de mejorar la efectividad del antiveneno y ampliar su cobertura.
dc.description.abstractenglishIntroduction: Snakebite accident is defined as the inoculation of a great variety of harmful components that cause pathophysiological disturbances that can trigger the death of the victim. The treatment of this type of envenomations involves the administration of antivenoms of equine origin, which provide direct or cross immunoreactivity against the venom of multiple species of snakes belonging to the same genus, however the presence of isoforms of some toxins of medical interest together with the low immunogenicity of some low molecular weight proteins, results in partial neutralization of the antivenoms and a suboptimal pharmacological response. In Colombia, the response of commercial antivenoms against the venom of poorly investigated snakes such as Micrurus medemi, Micrurus sangilensis and Micrurus helleri remains unknown. These snakes are endemic and widely distributed in some of the territories with the highest incidence of snakebite accidents, such as the Andean, Orinoco, and Amazon regions. Objective and methodology: The purpose of this study is to evaluate the immunorecognition efficacy of the polyvalent anticoral antivenom of the National Institute of Health against the venom of these three species, through affinity chromatography, enzyme-linked immunoadsorption assays and Western blot, with previous characterization of the venoms by electrophoresis and chromatography techniques. Results: The venoms of the three Micrurus snakes presented interspecific variations in physicochemical characteristics, such as molecular weight, isoelectric point, and grade of hydrophobicity. These variations influenced the immunoreactivity capacity of the polyvalent anticoral antivenom produced by the National Institute of Health. Although the antivenom showed cross-reactivity with the venoms of M. helleri, M. medemi and M. sangilensis, variable recognition towards low molecular weight toxins was observed. This hightlight the importance of conducting additional studies in snakes of the genus Micrurus in which their degree of reactivity is unknown, especially in regions with a high incidence of bites, to improve the effectiveness of the antivenom and expand its coverage.
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias Básicas Biomédicasspa
dc.description.sponsorshipInstituto Nacional de Salud
dc.format.mimetypeapplication/pdf
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/12851
dc.language.isoes
dc.publisher.facultyFacultad de Medicinaspa
dc.publisher.grantorUniversidad El Bosquespa
dc.publisher.programMaestría en Ciencias Básicas Biomédicasspa
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dc.rightsAttribution-NonCommercial-ShareAlike 4.0 Internationalen
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.localAcceso abiertospa
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectMicrurus medemi
dc.subjectMicrurus sangilensis
dc.subjectMicrurus helleri
dc.subjectVeneno
dc.subjectAntiveneno
dc.subject.keywordsMicrurus medemi
dc.subject.keywordsMicrurus sangilensis
dc.subject.keywordsMicrurus helleri
dc.subject.keywordsVenom
dc.subject.keywordsAntivenom
dc.subject.nlmW 50
dc.titleNEUTRALIZACIÓN DE LOS VENENOS DE SERPIENTES CORALES Micrurus medemi, Micrurus sangilensis y Micrurus helleri POR EL ANTIVENENO ANTICORAL DEL INSTITUTO NACIONAL DE SALUD
dc.title.translatedNeutralization of Coral Snake Venoms Micrurus medemi, Micrurus sangilensis, and Micrurus helleri by the Anticoral Antivenom of the National Institute of Health
dc.type.coarhttps://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttps://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersion
dc.type.localTesis/Trabajo de grado - Monografía - Maestríaspa

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