humanized mice for the study of dengue disease pathogenesis: biological assays

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dc.contributor.authorGutiérrez-Barbosa, Hernando
dc.contributor.authorMedina-Moreno, Sandra
dc.contributor.authorDavis, Harry
dc.contributor.authorBryant, Joseph
dc.contributor.authorChua, Joel
dc.contributor.orcidZapata, Juan Carlos [https://orcid.org/0000-0003-1931-6147]
dc.date.accessioned2022-02-09T20:05:38Z
dc.date.available2022-02-09T20:05:38Z
dc.date.issued2022
dc.description.abstractDengue is one of the most prevalent infectious diseases around the world, present in all continents and mainly affecting developing countries. With few tools to fight and study this disease, it is imperative to have reliable animal models that not only recapitulate human disease but also contain human components to understand the pathogenic mechanism and immune responses, allowing the development of new treatments and vaccines against dengue. Humanized mice are a significant advance in the development of in vivo models to understanding the relation of the human immune system and target organs such as the liver during the infection by dengue virus, allowing basic and preclinical research. In this chapter, we describe the use of humanized NSG mice (huNSG) for the study of dengue disease. The first model describes reconstitution of the human immune system by transplanting human CD34+ stem cells in newborn or adult NSG mice. The second model combines the reconstitution with CD34+ stem cells with the transplant of human primary hepatocytes. This dual reconstituted animal will have two of the major players involved in the development of dengue infection. However, there are still more biological components missing in this model for dengue, but researchers continue working to improve the huNSG model to reconstitute other human components.spa
dc.description.abstractenglishDengue is one of the most prevalent infectious diseases around the world, present in all continents and mainly affecting developing countries. With few tools to fight and study this disease, it is imperative to have reliable animal models that not only recapitulate human disease but also contain human components to understand the pathogenic mechanism and immune responses, allowing the development of new treatments and vaccines against dengue. Humanized mice are a significant advance in the development of in vivo models to understanding the relation of the human immune system and target organs such as the liver during the infection by dengue virus, allowing basic and preclinical research. In this chapter, we describe the use of humanized NSG mice (huNSG) for the study of dengue disease. The first model describes reconstitution of the human immune system by transplanting human CD34+ stem cells in newborn or adult NSG mice. The second model combines the reconstitution with CD34+ stem cells with the transplant of human primary hepatocytes. This dual reconstituted animal will have two of the major players involved in the development of dengue infection. However, there are still more biological components missing in this model for dengue, but researchers continue working to improve the huNSG model to reconstitute other human components.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1007/978-1-0716-1879-0_19
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1064-3745
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/6776
dc.language.isoeng
dc.publisherHumana Press Inc.spa
dc.publisher.journalMethods in Molecular Biologyspa
dc.relation.ispartofseriesMethods in Molecular Biology, 1064-3745, Vol 2409, 2022, pag 271-289spa
dc.relation.urihttps://link.springer.com/protocol/10.1007%2F978-1-0716-1879-0_19
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.localAcceso abiertospa
dc.subjectModelos de ratones con denguespa
dc.subjectRatones humanizadosspa
dc.subjectRespuesta inmunespa
dc.subjectPatogénesisspa
dc.subjectVacunasspa
dc.subjectDesarrollo de fármacosspa
dc.subject.keywordsDENVspa
dc.subject.keywordsDengue mouse modelsspa
dc.subject.keywordsHumanized micespa
dc.subject.keywordsImmune responsespa
dc.subject.keywordsPathogenesisspa
dc.subject.keywordsVaccinespa
dc.subject.keywordsDrug developmentspa
dc.titlehumanized mice for the study of dengue disease pathogenesis: biological assaysspa
dc.title.translatedhumanized mice for the study of dengue disease pathogenesis: biological assaysspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.coarversionhttps://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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