Fluorometric cell-ELISA for quantifying rabies infection and heparin inhibition

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dc.contributor.authorRincón, Verónica
dc.contributor.authorCorredor Figueroa, Adriana Patricia
dc.contributor.authorMarlen Martinez-Gutierrez
dc.contributor.authorCastellanos, Jaime
dc.date.accessioned2021-02-23T16:17:52Z
dc.date.available2021-02-23T16:17:52Z
dc.date.issued2005
dc.description.abstractenglishThe purpose of the present study was to implement a fluorometric method for detecting and quantifying viral antigens in human meduloblas-toma cells infected by two types of fixed rabies virus (CVS-MB and CVS-BHK) and a street virus using a cell-enzyme linked immunosorbentassay (cell-ELISA) technique; alkaline phosphatase was used as the antibody-marker enzyme and 4-methyl-umbelliferyl-phosphate as thefluorogenic substrate. The system was used for detecting up to 1:10,000 viral inoculums, followed by evaluating the effect of heparin oninfection. Infected cultures were reliably differentiated from their respective negative controls in both assays allowing data to be analysedstatistically. As reported in another study, heparin produces strong inhibition when the CVS-BHK viral strain is used for infection; it has thusbeen suggested that it binds to the neural cell adhesion molecule and could be blocked by using this drug. This fluorometric method is lesstime-consuming, has increased reproducibility and useful for quantitation of collected data and can therefore be considered as a useful toolfor research.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.jviromet.2005.03.002
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1879-0984
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlhttps://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/5421
dc.language.isoeng
dc.publisherElsevierspa
dc.publisher.journalJournal of virological methodsspa
dc.relation.ispartofseriesJournal of virological methods, 1879-0984, Vol. 127, Nro. 1, 2005, p. 33-39spa
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0166093405000832?via%3Dihub
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2005-04-07
dc.rights.localAcceso abiertospa
dc.subject.keywords4-Methyl-umbelliferyl-phosphate (MUP)spa
dc.subject.keywordsCell-ELISAspa
dc.subject.keywordsFluorometric methodspa
dc.subject.keywordsHeparinspa
dc.subject.keywordsHuman meduloblastoma cellsspa
dc.subject.keywordsRabies virusspa
dc.titleFluorometric cell-ELISA for quantifying rabies infection and heparin inhibitionspa
dc.title.translatedFluorometric cell-ELISA for quantifying rabies infection and heparin inhibitionspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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