Eikenella corrodens lipopolysaccharide stimulates the pro-atherosclerotic response in human coronary artery endothelial cells and monocyte adhesion

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dc.contributor.authorViafara García, Sergio Marino
dc.contributor.authorGualtero, Diego Fernando
dc.contributor.authorAvila‐Ceballos, Daniel
dc.contributor.authorLafaurie, Gloria Ines
dc.contributor.orcidLafaurie, Gloria Ines [0000-0003-3986-0625]
dc.contributor.orcidGualtero, Diego Fernando [0000-0002-6407-5605]
dc.contributor.orcidViafara García, Sergio Marino [0000-0002-4208-1422]
dc.date.accessioned2020-05-18T06:54:36Z
dc.date.available2020-05-18T06:54:36Z
dc.date.issued2018
dc.description.abstractenglishEikenella corrodens is a gram‐negative bacterium, and although primarily associated with periodontal infections or infective endocarditis, it has been identified in coronary atheromatous plaques. The effect of its lipopolysaccharide (LPS) on human coronary artery endothelial cells (HCAECs) is unknown. Our aim was to examine the mechanism underlying the inflammatory response in HCAECs stimulated with E. corrodens‐LPS and to evaluate monocyte adhesion. Endothelial responses were determined by measuring the levels of chemokines and cytokines using flow cytometry. The surface expression of intercellular adhesion molecule 1 (ICAM‐1) was determined using a cell‐based ELISA, and the adhesion of THP‐1 monocytes to HCAECs was also monitored. The involvement of toll‐like receptors (TLRs) 2 and 4 was examined using TLR‐neutralizing antibodies, and activation of extracellular signal‐regulated kinase (ERK)1/2 and nuclear factor‐kappa B (NF‐κB) p65 were measured by western blotting and ELISA, respectively. Eikenella corrodens‐LPS increased secretion of interleukin‐8 (IL‐8), monocyte chemotactic protein 1 (MCP‐1), and granulocyte–macrophage colony‐stimulating factor (GM‐CSF), and expression of ICAM‐1 on the surface of HCAECs, consistent with the increased adhesion of THP‐1 cells. Moreover, E. corrodens‐LPS interacted with TLR4, a key receptor able to maintain the levels of IL‐8, MCP‐1, and GM‐CSF in HCAECs. Phosphorylation of ERK1/2 and activation of NF‐κB p65 were also increased. The results indicate that E. corrodens‐LPS activates HCAECs through TLR4, ERK, and NF‐κB p65, triggering a pro‐atherosclerotic endothelial response and enhancing monocyte adhesion.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/eos.12580
dc.identifier.issn1600-0722
dc.identifier.urihttps://hdl.handle.net/20.500.12495/2905
dc.language.isoeng
dc.publisherWileyspa
dc.publisher.journalEuropean Journal of Oral Sciencesspa
dc.relation.ispartofseriesEuropean Journal of Oral Sciences, 1600-0722, Vol 126, Num 6, 2018, pag 476-484spa
dc.relation.urihttps://onlinelibrary.wiley.com/doi/abs/10.1111/eos.12580
dc.rights.creativecommons2018
dc.rights.localAcceso cerradospa
dc.subject.decsEnfermedades periodontalesspa
dc.subject.decsLeucocitos mononuclearesspa
dc.subject.decsInmunidad innataspa
dc.subject.keywordsAtherosclerosisspa
dc.subject.keywordsCoronary artery diseasespa
dc.subject.keywordsInnate immunityspa
dc.subject.keywordsMonocyte/macrophagespa
dc.subject.keywordsPeriodontitisspa
dc.titleEikenella corrodens lipopolysaccharide stimulates the pro-atherosclerotic response in human coronary artery endothelial cells and monocyte adhesionspa
dc.title.translatedEikenella corrodens lipopolysaccharide stimulates the pro-atherosclerotic response in human coronary artery endothelial cells and monocyte adhesionspa
dc.typearticlespa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localartículospa

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