Estudio de la Miosina C de Plasmodium falciparum (PfMYO-C)

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dc.contributor.advisorChaparro Olaya, Jacqueline
dc.contributor.authorAponte Briceño, Samanda Lizbeth
dc.date.accessioned2023-03-02T15:06:20Z
dc.date.available2023-03-02T15:06:20Z
dc.date.issued2011
dc.description.abstractDe las cinco especies de Plasmodium que causan la malaria, P. falciparum es la que produce la mayor mortalidad en el mundo, a pesar de los esfuerzos de los países endémicos por implementar diferentes estrategias para su control y potencial erradicación. El conocimiento de los procesos biológicos de P. falciparum es una herramienta clave para la postulación de blancos terapéuticos o blancos para vacunas. Las miosinas son proteínas motoras que participan en muchos de estos procesos en las células eucariotas y hasta el momento en P. falciparum se han identificado seis secuencias que codifican para miosinas y se han caracterizado cinco durante el ciclo intraeritrocitario. Por su transcripción, localización y función, dos de estas miosinas se han visto potencialmente involucradas en el proceso de invasión (Pfmyo-A y Pfmyo-B) mientras que las otras tres (Pfmyo-D, Pfmyo-E y Pfmyo-F) al parecer se encuentran actuando en procesos diferentes aún desconocidos. Pfmyo-C es la única miosina de P. falciparum que aún no se ha caracterizado; sinembargo de esta proteína se conoce su patrón de transcripción y varias características particulares en su secuencia, en especial la presencia de repeticiones de tipo WD40 que sugieren que esta miosina puede estar involucrada en procesos diferentes al de la invasión. El objetivo de este trabajo fue realizar un acercamiento a la caracterización de Pfmyo-C durante el ciclo intraeritrocitario en cuanto a su transcripción, expresión y localización, así como analizar su secuencia empleando herramientas bioinformáticas con el fin de inferir la posible función de esta proteína en P. falciparum. Los resultados obtenidos mediante RT-PCR muestran que la expresión de mARN de Pfmyo-C se da principalmente en el estadío de trofozoitos maduros y esquizontes tempranos, y este patrón correlaciona directamente con la expresión de Pfmyo-C en el estadío de esquizonte temprano determinada por western-blot empleando sueros policlonales obtenidos a partir de ratones inoculados con una proteína recombinante para Pfmyo-C. No obstante, el tamaño detectado de la proteína no correlaciona con el tamaño esperado (250KDa), lo que nos hace sugerir la presencia de un intrón no predicho en las bases de datos, o la existencia de una modificación o procesamiento de la proteína. Con base en los análisis de dominios de Pfmyo-C, podemos sugerir de manera muy preliminar que esta proteína puede estar involucrada en procesos de transducción de señales durante las últimas etapas de desarrollo del parásito dentro del glóbulo rojo.spa
dc.description.abstractenglishIn spite of the efforts made in order to implement strategies for its control and potential eradication, Malaria remains the most important vector-borne infectious disease. Currently, it is worldwide accepted that knowledge of the biological processes of Plasmodium falciparum is a key tool for the postulation of therapeutic targets and vaccines candidates. Myosins are molecular motors involved in many processes for a range of functions like muscle contraction, vision, hearing, and host cell invasion of Apicomplexa, phylum which comprises unicellular eukaryotes that live as obligate intracellular parasites and includes important pathogens such as Plasmodium. Sequence encoding six „true‟ myosin genes is present in the genome of P. falciparum and until today, five of these myosins have been characterized during the red blood cell cycle of the parasite. Due to their pattern of transcription, location and expression, two of these myosins are apparently involved in the invasion process (Pfmyo-A and Pfmyo-B) whereas the other three (Pfmyo-D, Pfmyo-E and Pfmyo-F) do not. Since Pfmyo-C is the only one myosin not characterized in P. falciparum, the aim of this project was to define the transcription and expression patterns as well as the cellular localization of Pfmyo-C along the intra red blood cell cycle of the parasite. We also analyzed Pfmyo-C sequence using bioinformatics with the purpose of inferring possible functions of the protein. The results show that the transcription of the pfmyo-c gene occurs mainly in the stage of mature trophozoites and early schizonts, and this pattern correlates directly with the specific expression of Pfmyo-C in the early schizont stage. However, the size of the protein detected is smaller than the expected one (250KDa) and that suggests the presence of a non-predicted intron or a potential modification of the protein. Finally, the bioinformatics analysis let us to suggest that Pfmyo-C can be involved in signal transduction processes during the last stages of the asexual cycle of the parasite.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias Básicas Biomédicasspa
dc.format.mimetypeapplication/pdf
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/10091
dc.language.isospa
dc.publisher.facultyFacultad de Medicinaspa
dc.publisher.grantorUniversidad El Bosquespa
dc.publisher.programMaestría en Ciencias Básicas Biomédicasspa
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dc.rights.accessrightsinfo:eu-repo/semantics/closedAccess
dc.rights.accessrightshttps://purl.org/coar/access_right/c_14cb
dc.subjectMiosina Cspa
dc.subjectPlasmodium falciparumspa
dc.subjectBlancos terapéuticosspa
dc.subjectBlancos para vacunasspa
dc.subject.keywordsMyosin Cspa
dc.subject.keywordsPlasmodium falciparumspa
dc.subject.keywordsTherapeutic Targetsspa
dc.subject.keywordsVaccine Targetsspa
dc.subject.nlmW 50
dc.titleEstudio de la Miosina C de Plasmodium falciparum (PfMYO-C)spa
dc.title.translatedPlasmodium falciparum myosin C (PfMYO-C) studyspa
dc.type.coarhttps://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttps://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersion
dc.type.localTesis/Trabajo de grado - Monografía - Maestríaspa

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