Genomic and molecular characterization of clinical isolates of Enterobacteriaceae harboring mcr-1 in Colombia, 2002 to 2016

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dc.contributor.authorSaavedra, Sandra Yamile
dc.contributor.authorDiaz, Lorena
dc.contributor.authorWiesner, Magdalena
dc.contributor.authorArévalo, Stefany Alejandra
dc.contributor.authorCorrea, Adriana
dc.contributor.authorReyes, Jinnethe
dc.contributor.authorHidalgo, Andrea Melissa
dc.contributor.authorde la Cadena, Elsa
dc.contributor.authorPerenguez, Marcela Nataly
dc.contributor.authorMontaño, Lucy Angeline
dc.contributor.authorArdila, Javier
dc.contributor.authorRíos, Rafael
dc.contributor.authorOvalle, María Victoria
dc.contributor.authorDíaz, Paula
dc.contributor.authorPorras, Paola
dc.contributor.authorVillegas, María Virginia
dc.contributor.authorArias, Cesar A
dc.contributor.authorBeltrán, mauricio
dc.contributor.authorDuarte, Carolina
dc.contributor.orcidde la Cadena, Elsa [0000-0003-0361-7893]
dc.contributor.orcidVillegas, María Virginia [0000-0003-1898-9067]
dc.date.accessioned2020-05-13T19:29:04Z
dc.date.available2020-05-13T19:29:04Z
dc.date.issued2017
dc.description.abstractenglishPolymyxins are last-resort antimicrobial agents used to treat infections caused by carbapenem-resistant Enterobacteriaceae. Due to the worldwide dissemination of polymyxin resistance in animal and human isolates, we aimed to characterize polymyxin resistance associated with the presence of mcr-1 in Enterobacteriaceae and nonfermenter Gram-negative bacilli, using isolates collected retrospectively in Colombia from 2002 to 2016. A total of 5,887 Gram-negative clinical isolates were studied, and 513 were found to be resistant to the polymyxins. Susceptibility to colistin was confirmed by broth microdilution for all mcr-1-positive isolates, and these were further subjected to whole-genome sequencing (WGS). The localization of mcr-1 was confirmed by S1 pulsed-field gel electrophoresis (S1-PFGE) and CeuI-PFGE hybridization. Transferability was evaluated by mating assays. A total of 12 colistin-resistant isolates recovered after 2013 harbored mcr-1, including 8 Escherichia coli, 3 Salmonella enterica serovar Typhimurium, and 1 Klebsiella pneumoniae isolate. E. coli isolates were unrelated by PFGE and belonged to 7 different sequence types (STs) and phylogroups. S. Typhimurium and K. pneumoniae isolates belonged to ST34 and ST307, respectively. The mcr-1 gene was plasmid borne in all isolates but two E. coli isolates which harbored it on the chromosome. Conjugation of mcr-1 was successful in 8 of 10 isolates (8.2 × 10−5 to 2.07 × 10−1 cell per recipient). Plasmid sequences showed that the mcr-1 plasmids belonged to four different Inc groups (a new IncP-1 variant and the IncFII, IncHI1, and IncH families). Our results indicate that mcr-1 is circulating in clinical isolates of colistin-resistant Enterobacteriaceae in Colombia and is mainly harbored in transferable plasmids.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1128/AAC.00841-17
dc.identifier.issn1098-6596
dc.identifier.urihttps://hdl.handle.net/20.500.12495/2701
dc.language.isoeng
dc.publisherAmerican Society for Microbiologyspa
dc.publisher.journalAntimicrobial Agents and Chemotherapyspa
dc.relation.ispartofseriesAntimicrobial Agents and Chemotherapy, 1098-6596, Vol. 61, Nro. 12, 2017, p. 1-13spa
dc.relation.urihttps://aac.asm.org/content/61/12/e00841-17
dc.rights.creativecommons2017
dc.rights.localAcceso cerradospa
dc.subject.decsAntiinfecciososspa
dc.subject.decsPolimixinasspa
dc.subject.decsPaenibacillus polymyxaspa
dc.subject.keywordsGenomicspa
dc.subject.keywordsmcr-1spa
dc.subject.keywordsEnterobacteriaceaespa
dc.subject.keywordsColombiaspa
dc.titleGenomic and molecular characterization of clinical isolates of Enterobacteriaceae harboring mcr-1 in Colombia, 2002 to 2016spa
dc.typearticlespa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localartículospa

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