In vitro antiviral activity of lactobacillus casei and bifidobacterium adolescentis against rotavirus infection monitored by NSP 4 protein production

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dc.contributor.authorOlaya Galán, N.N.
dc.contributor.authorUlloa Rubiano, J.C.
dc.contributor.authorVelez Reyes, F.A.
dc.contributor.authorFernandez Duarte, K.P.
dc.contributor.authorSalas Cárdenas, S.P.
dc.contributor.authorGutierrez Fernandez, M.F.
dc.date.accessioned2020-08-06T17:45:19Z
dc.date.available2020-08-06T17:45:19Z
dc.date.issued2016
dc.description.abstractenglishAims The aim of this study was to determine the antiviral activity of four probiotic metabolites (Lactobacillus and Bifidobacetrium species) against rotavirus in vitro infection monitored by the NSP 4 protein production and Ca2+ release. Methods and Results The antiviral effect of the metabolites was performed due a comparison between a blocking model and an intracelullar model on MA 104 cells, with the response of NSP 4 production and Ca2+ liberation measured by flow cytometry. Significant results were obtained with the metabolites of Lactobacillus casei, and Bifidobacterium adolescentis in the reduction of the protein production (P = 0·04 and P = 0·014) and Ca2+ liberation (P = 0·094 and P = 0·020) in the intracellular model, which suggests a successful antiviral activity against RV infection. Conclusions This study demonstrates that probiotic metabolites were able to interfere with the final amount of intracellular NSP 4 protein and a successful Ca2+ regulation, which suggests a new approach to the mechanism exerted by probiotics against the rotavirus infection. Significance and Impact of the Study A novel anti‐rotaviral effect exerted by probiotic metabolites monitored by the NSP 4 protein during the RV in vitro infection and the effect on the Ca2+ release is reported; suggesting a reduction on the impact of the infection by decreasing the damage of the cells preventing the electrolyte loss.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/jam.13069
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1365-2672
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlhttps://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/3705
dc.language.isoeng
dc.publisherWileyspa
dc.publisher.journalJournal of applied Microbiologyspa
dc.relation.ispartofseriesJournal of applied Microbiology, 1365-2672, Vol. 120, Nro. 4, 2016, p. 1041-1051spa
dc.relation.urihttps://sfamjournals-onlinelibrary-wiley-com.ezproxy.unbosque.edu.co/doi/full/10.1111/jam.13069
dc.rightsAttribution 4.0 International*
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2016-04
dc.rights.localAcceso abiertospa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.keywordsAntiviral effectspa
dc.subject.keywordsBifidobacterium adolescentisspa
dc.subject.keywordsLactobacillus caseispa
dc.subject.keywordsNSP 4 proteinspa
dc.subject.keywordsProbiotic metabolitesspa
dc.subject.keywordsRotavirusspa
dc.titleIn vitro antiviral activity of lactobacillus casei and bifidobacterium adolescentis against rotavirus infection monitored by NSP 4 protein productionspa
dc.title.translatedIn vitro antiviral activity of lactobacillus casei and bifidobacterium adolescentis against rotavirus infection monitored by NSP 4 protein productionspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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