Evaluación in vitro de los agentes neurotóxicos liberados por las células de la microglía, infectadas con el virus dengue neuroadaptado (D4MB-6)

Vista sencilla de ítem
dc.contributor.advisorVelandia-Romero, Myriam Lucía
dc.contributor.advisorCastellanos, Jaime
dc.contributor.authorBeltrán Zúñiga, Edgar Orlando
dc.contributor.orcidCastellanos, Jaime [0000-0003-1596-8383]
dc.contributor.orcidVelandia-Romero, Myriam Lucía [0000-0002-3340-7304]
dc.date.accessioned2021-02-22T21:21:43Z
dc.date.available2021-02-22T21:21:43Z
dc.date.issued2021
dc.description.abstractLa infección causada por el virus del dengue (DENV) se ha relacionado con el desarrollo de alteraciones neurológicas y comportamentales en humanos. Dicha asociacione se ha confirmado por el desarrollo de signos claros de alteración neurológica durante y después de la infección y por la presencia de proteínas y/o RNA viral en líquido cefaloraquídeo o en muestras de tejido nervioso obtenidas post morten. Esto sugiere que el virus puede invadir el Sistema Nervioso Central (SNC). Por la dificultad de reproducir los signos y síntomas del dengue en animales, nuestro grupo desarrolló un modelo in vivo de neuroinfección en el cual ratones neonatos se inocularon a nivel periférico con una cepa de virus adaptada a tejido nervioso (D4MB-6). Como resultado, se observó que la variante viral logró invadir el SNC e infectó algunas poblaciones celulares como neuronas, oligodendrocitos y células de la microglía. Estas últimas presentaron evidentes cambios morfológicos que sugerían activación. Adicionalmente se observó que en el tejido nervioso de animales infectados la infección indujo una exacerbada respuesta inmune y la excitotoxicidad por glutamato, espectos que en conjunto causaron la muerte neuronal y el daño en la arquitectura tisular. Sin embargo, la población celular del tejido nervioso que secreta estas moléculas no ha sido identificada aún. Experimentalmente se ha demostrado que en diferentes patologías -incluso virales- la principal fuente de citoquinas pro-inflamatorias, glutamato y otros agentes provienen de las células de la microglía infectadas y/o activadas. Todos los agentes producidos y liberados por estas células, pueden de manera conjunta o independiente ocasionar el daño y la muerte neuronal. Por lo tanto, en este trabajo se evaluó en un cultivo primario de células de la microglía de ratón lactante, su susceptibilidad a la infección con la variante adaptada -D4MB-6- o no a tejido nervioso y algunos aspectos relacionados con su activación. Como resultado se observó que las células microgliales presentaron una alta susceptibilidad a la infección y en los tiempos post-infección evaluados presentaron altas tasas de proliferación y supervivencia. Adicionalmente se observó que las células presentaron cambios morfológicos y produjeron algunas moléculas pro-inflamatorias y glutamato, que indujeron a las células a sostener en los tiempos evaluados un estado de activación que fue modulado y regulado por los fármacos VPA o MK-801. En conjunto estos resultados permitieron describir que la infección por DENV indujo la activación y producción de agentes pro-inflamatorios y glutamato por parte de las células de la microglía, por lo tanto, sugerimos que estas células son centrales en el desarrollo de la nueuroinfección y neuropatogenia causada por el DENV.spa
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias Básicas Biomédicasspa
dc.format.mimetypeapplication/pdf
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/5409
dc.publisher.facultyFacultad de Medicinaspa
dc.publisher.grantorUniversidad El Bosquespa
dc.publisher.programMaestría en Ciencias Básicas Biomédicasspa
dc.relation.referencesAkiyama, H. Barger, S. Barnum, S. Bradt, B. Bauer, J. Cole, G. et al. (2000). Inflammation and Alzheimer’s disease. Neurobiol Aging. 21:383–421.spa
dc.relation.referencesAlliot F, Marty MC, Cambier D, Pessac B. (1996). A spontaneously immortalised mouse microglial cell line expressing CD4. Brain Res Dev Brain Res. 95:140–3.spa
dc.relation.referencesAloisi F. (2001). Immune function of microglia. Glia. 36:165–79.spa
dc.relation.referencesAraújo, F. Nogueira, R. De Sousa -Araújo, M. Perdigão, A. Cavalcanti, et al. (2012). Dengue in Patients with Central Nervous System Manifestations, Brazil. Emerg Infect Dis. 18(4): 677–79.spa
dc.relation.referencesBal-Price, A. Moneer, Z. Brown, G. (2002). Nitric oxide induces rapid, calciumdependent release of vesicular glutamate and ATP from cultured rat astrocytes. Glia. 40(3): 312–23.spa
dc.relation.referencesBaloul, L. Lafon, M. (2003). Apoptosis and rabies virus neuroinvasion. Biochimie. 85(8):777-88.spa
dc.relation.referencesBarger SW, Hörster D, Furukawa K, Goodman Y, Krieglstein J, Mattson M. (1995). Tumor necrosis factors alpha and beta protect neurons against amyloid beta-peptide toxicity: evidence for involvement of a kappa B-binding factor and attenuation of peroxide and Ca2 accumulation. Proc Natl Acad Sci USA. 92(20):9328-32.spa
dc.relation.referencesBarres, B. (2008). The mystery and magic of glia: A perspective on their roles in health and disease. Neuron. 60(3):430–40.spa
dc.relation.referencesBaud, V. Karin, M. (2001). Signal transduction by tumor necrosis factor and its relatives. Trends Cell Biol. 11(9):372–77.spa
dc.relation.referencesBhatt, S. Gething, P. Brady, O. Messina, J. Farlow, A. Moyes, C. et al. (2013). The global distribution and burden of dengue. Nature. 496: 504–07.spa
dc.relation.referencesBezzi, P. Carmignoto, G. Pasti, L. Vesce, S. Rossi, D. Rizzini P. et al. (1998). Prostaglandins stimulate calciumdependent glutamate release in astrocytes. Nature. 391: 281-85.spa
dc.relation.referencesBoche, D. Perry, V. Nicoll, J. (2013). Review: activation patterns of microglia and their identification in the human brain. Neuropathol Appl Neurobiol. 39(1): 3–18.spa
dc.relation.referencesBode, K. Schroder, K. Hume, D. Ravasi, T. Heeg, K. Sweet, M. et al. (2007). Histone deacetylase inhibitors decrease Toll-like receptor-mediated activation of proinflammatory gene expression by impairing transcription factor recruitment. Immunol. 122: 596-606.spa
dc.relation.referencesBoje, K. Arora, P. (1992). Microglial produced nitrid oxide and nitrogen oxides mediate neuronal cells. Brain Res. 587(2): 250 – 56.spa
dc.relation.referencesBoscia, F. Esposito, C. Casamassa, A. De Franciscis, V. Annunziato, L. Cerchia, L. (2013). The isolectin IB4 binds RET receptor tyrosine kinase in microglia. J Neurochem. 126(4): 428-36.spa
dc.relation.referencesCabrera, J. Lopes, O. Hernández, E. (1984). Alteraciones electroencefalográficas en la fiebre hemorrágica de dengue. Revisión sobre sus manifestaciones neurológicas. Rev Cub Med. 23: 468-78.spa
dc.relation.referencesCamacho, S. (2015). Evaluación de la Excitotoxicidad por glutamato inducida por el virus dengue neuroadaptado D4MB-6 (Tesis de Maestría). Universidad El Bosque. Bogotá- Colombia.spa
dc.relation.referencesCarlson, N. Wieggel, W. Chen, J. Bacchi, A. Rogers, S. Gahring, L. (1999). Inflammatory cytokines IL-1 alpha, IL-1 beta, IL-6, and TNF-alpha impart neuroprotection to an excitotoxin through distinct pathways. J Immunol. 163(7): 3963-8.spa
dc.relation.referencesCarod-Artal, F. Wichmann, O. Farrar, J. Gascón, J. (2013). Neurological complications of dengue virus infection. Lancet Neurol. 12(9): 906–19.spa
dc.relation.referencesCarson, M. (2002). Microglia as liaisons between the immune and central nervous systems, functional implications for multiple sclerosis. Glia. 40:218–31.spa
dc.relation.referencesCarson MJ, Reilly CR, Sutcliffe JG, Lo D. (1998). Mature microglia resemble immature antigen presenting cells. Glia. 22:72–85.spa
dc.relation.referencesChambers, T. Hahn, C. Galler, R. Rice, C. (1990). Flavivirus genome organization, expression, and replication. Annu Rev Microbiol. 44:649–688.spa
dc.relation.referencesChao, C. Hu, S. Peterson, P. (1995). Glia, cytokines, and neurotoxicity. Crit Rev Neurobiol. 9(2-3):189-205.spa
dc.relation.referencesChao, C. Hu, S. Peterson, P. (1996). Glia: the not so innocent bystanders. J Neurovirol. 2(4):234-39.spa
dc.relation.referencesChen, C. Ou, Y. Chang, C. Pan, H. Liao, S. Chen, S. et al. Raung SL, Lai C. (2012). Glutamate released by Japanese encephalitis virus infected microglia involves TNF-α signaling and contributes to neuronal death. Glia. 60(3):487501.spa
dc.relation.referencesChéret, C. Gervais, A. Lelli, A. Colin, C. Amar, L. Ravassard, P. et al. (2008). Neurotoxic activation of microglia is promoted by a Nox1-dependent NADPH oxidase. J. Neurosci. 28(46): 12039–51.spa
dc.relation.referencesChhor, V. Le Charpentier, T. Lebon, S. Oré, M. Celador, I. Josserand, J. et al. (2013). Characterization of phenotype markers and neuronotoxic potential of polarized primary microglia in vitro. Brain Behav Immun. 32:70–85.spa
dc.relation.referencesDe Groot, C. Montagne, L. Janssen, I. Ravid, R. Van, D. Veerhuis, V. (2000). Isolation and characterization of adult microglial cells and oligodendrocytes derived from postmortem human brain tissue. Brain Res Brain Res Protoc. 5:85–94.spa
dc.relation.referencesDeierborg, T. (2013). Preparation of primary microglia cultures from postnatal mouse and rat brains. Methods Mol Biol. 1041:25-31.spa
dc.relation.referencesDevarajan, G. Chen, M. Muckersie, E. Xu, H. (2014). Culture and characterization of microglia from the adult murine retina. Scientific World Journal. 2014:894-368.spa
dc.relation.referencesDrapier, J. Hibbs, J Jr (1986): Murine cytotoxic activated macrophages inhibit aconitase in tumor cells. Inhibition involves the iron-sulfur prosthetic group and is reversible. J Clin Invest. 78:790–797.spa
dc.relation.referencesEstrada, R. (1983). Sobre los síndromes neurológicos que han ocurrido durante nuestras dos recientes epidemias por virus dengue y sus posibles interrelaciones. Rev Cub Hig Epid. 21:105-113.spa
dc.relation.referencesEugenin, E. Dyer, G. Calderon, T. Berman, J. (2005). HIV-1 tat protein induces a migratory phenotype in human fetal microglia by a CCL2 (MCP1)- dependent mechanism: possible role in NeuroAIDS. Glia. 49(4):501-10.spa
dc.relation.referencesFenn, A. Henry. C. Huang, Y. Dugan, A. Godbout, J. (2012). Lipopolysaccharide- induced interleukin (IL) 24 receptor-alpha expression and corresponding sensitivity to the M2 promoting effects of IL-4 are impaired in microglia of aged mice. Brain Behav Immun. 26:766–777.spa
dc.relation.referencesFine, S. Angel, R. Perry, S. Epstein, L. Rothstein, J. Dewhurst, S. et al. (1996) Tumor necrosis factor inhibits glutamate uptake by primary human astrocytes. Implications for pathogenesis of HIV-1 dementia. J. Biol. Chem. 271, 1530306.spa
dc.relation.referencesFlaris, N. Densmore, T. Molleston, C. Hickey, W. (1993). Characterization of microglia and macrophages in the central nervous system of rats: Definition of the differential expression of molecules using standard and novel monoclonal antibodies in normal CNS and in four models of parenchymal reaction. Glia. 7:34-40.spa
dc.relation.referencesFord, A. Goodsall, A. Hickey, W. Sedgwick, J. (1995). Normal adult ramified microglía separated from other central nervous system macrophages by flow cytometric sorting. Phenotypic differences defined and direct ex vivo antigen presentation to myelin basic protein-reactive CD4+T cells compared. J Immunol. 154:4309–21.spa
dc.relation.referencesGanter, S. Northoff, H. Mannel, D. Gebicke-Harter, P. (1992). Growth control of cultured microglia. J Neurosci Res. 33:218–230.spa
dc.relation.referencesGao, H. Hong, J. (2008). Why neuro-degenerative diseases are progressive: uncontrolled inflammation drives disease progression. Trends Immunol. 29(8):357-65spa
dc.relation.referencesGarcía-Rivera, E. & Rigau-Perez, J. (2002). Encephalitis and dengue. Lancet. 360(9328): 261.spa
dc.relation.referencesGendelman, H. Orenstein, J. Martin, M. Ferrua, C. Mitra, R. Phipps, T. et al. (1998). Efficient isolation and propagation of human immunodeficiency virus on recombinant colony-stimulating factor 1-treated monocytes. J Exp Med. 167:1428-1441.spa
dc.relation.referencesGeorg, B. Morwinsky, A. Keitel, V. Qvartskhava, N. Schreor, K. Heaussinger D. (2010). Ammonia triggers exocytotic release of L-glutamate from cultured rat astrocytes. Glia. 58:691–705.spa
dc.relation.referencesGerhauser, I. Hansmann, F. Puff, C. Kumnok, J. Schaudien, D. Wewetzer, K. et al. (2012). Theiler's murine encephalomyelitis virus induced phenotype switch of microglia in vitro. Neuroimmunol. 252(1-2):49-55.spa
dc.relation.referencesGhoshal, A. Das, S. Ghosh, S. Mishra, M. Sharma, V. Koli, P. Sen, E. Basu, A. (2007). Proinflammatory mediators released by activated microglía induces neuronal death in Japanese encephalitis. Glia. 55:483–96.spa
dc.relation.referencesGiulian, D. (1993). Reactive glia as rivals in regulating neuronal survival. Glia. 7(1):102-10.spa
dc.relation.referencesGiulian, D & Baker, T. (1986). Characterization of ameboid microglia isolated from developing mammalian brain. J Neurosci. 6(8):2163-78.spa
dc.relation.referencesGlass, C. Saijo, K. Winner, B. Marchetto, M. Gage, F. (2010). Mechanisms underlying inflammation in neurodegeneration. Cell. 140 (6): 918–34.spa
dc.relation.referencesGörg, B. Morwinsky, A. Keitel, V. Qvartskhava, N. Schreor, K. Heaussinger D. (2010). Ammonia triggers exocytotic release of L-glutamate from cultured rat astrocytes. Glia. 58:691–705.spa
dc.relation.referencesGreenamyre, J. Porter, R. (1994). Anatomy and physiology of glutamate in the CNS. Neurology. 44:S7–S13.spa
dc.relation.referencesGubler, D. (2010). Dengue Viruses. In: Brian, W.J., Mahy Marc H.V Regenmortel, Van Regenmorter (Eds.), Desk Encyclopedia of Human and Medical Virology. Academic Press. 371–381.spa
dc.relation.referencesHanish, U. (2002). Microglia as a Source and Target of Cytokines. Glía. 40: 140-155.spa
dc.relation.referencesHanisch, U. Kettenmann, H. (2007). Microglia: active sensor and versatile effector cells in the normal and pathologic brain. Nat Neurosci. 10(11): 1387–94.spa
dc.relation.referencesHewett, S. Csernansky, C. Choi, D. (1994): Selective potentiation of NMDA- induced neuronal injury following induction of astrocytic iNOS. Neuron. 13:487–94.spa
dc.relation.referencesHickey, W. (2001). Basic principles of immunological surveillance of the normal central nervous system. Glia. 36:118–24.spa
dc.relation.referencesJeong, M. Hashimoto, R. Senatorov, V. Fujimaki, K. Ren, M. Lee, M. et al. (2003). Valproic acid, a mood stabilizer and anticonvulsant, protects rat cerebral cortical neurons from spontaneous cell death: a role of histone deacetylase inhibition. FEBS Lett. 542(1-3):74-78.spa
dc.relation.referencesJin, D. Lim, C. Hwang, J. Ha, I. Han, J. (2005). Anti-oxidant and antiinflammatory activities of macelignan in murine hippocampal cell line and primary culture of rat microglial cells. Biochem Biophys Res Commun. 331(4):1264-1269.spa
dc.relation.referencesKinouchi, K. Brown, G. Pasternak, G. Donner, D. (1991). Identification and characterization of receptors for tumor necrosis factor-alpha in the brain. Biochem Biophys Res Commun. 181(3):1532–38.spa
dc.relation.referencesKitamura, T. (1973). The origin of brain macrophages-some considerations on the microglia theory of Del Rio-Hortega. Acta Pathol Jpn. 23(1):11-26.spa
dc.relation.referencesKlegeris, A. McGeer, E. McGeer, P. (2007). Therapeutic approaches to inflammation in neurodegenerative disease. Curr Opin Neurol, 20(3):51–357.spa
dc.relation.referencesKotoh, K. Kato, M. Kohjima, M. Tanaka, M. Miyazaki, M. Nakamura, K. et al. (2011). Lactate dehydrogenase production in hepatocytes is increased at an early stage of acute liver failure. Exp Ther Med. 2(2):195-199.spa
dc.relation.referencesKrammer P. (2000). CD95's deadly mission in the immune system. Nature, 407(6805):789–795.spa
dc.relation.referencesKrishnan, C. Kaplin, A. Deshpande, D. Pardo, C. Kerr, D. Transverse myelitis: Pathogenesis, diagnosis and treatment. Front Biosci 2004; 9:1483-9.spa
dc.relation.referencesKumar, R. Tripathi, S. Tambe, J. Arora, V. Srivastava, A. Nag, V. (2008). Dengue encephalopathy in children in northern india: clinical features and comparison with non dengue. J Neurol Sci. 269(1-2):41–48.spa
dc.relation.referencesLee, S. Liu, W. Roth, P. Dickson, D. Berman, J. Brosnan, C. et at. (1993). Macrophage colony stimulating factor in human fetal astrocytes and microglia: differential regulation by cytokines and lipopolysaccharide, and modulation of class 11 MHC on microglia. J Immunol. 150:594-604.spa
dc.relation.referencesLiang, J. Takeuchi, H. Doi, Y. Kawanokuchi, J. Sonobe, Y. Jin, S. (2008). Excitatory amino acid transporter expression by astrocytes is neuroprotective against microglial excitotoxicity. Brain Res. 1210:11–19.spa
dc.relation.referencesLiao, S. Chen, C. (2001). Differential effects of cytokines and redox potential on glutamate uptake in rat cortical glial cultures. Neurosci Lett. 299:113–116.spa
dc.relation.referencesLipton, S. Choi, Y. Pan, Z. Lei, S. Chen, H. Sucher, N. (1993): A redox-based mechanism for the neuroprotective and neurodestructive effects of nitric oxide and related nitroso-compounds. Nature. 364:626–32.spa
dc.relation.referencesLiu, G. Nagarajah, R. Banati, R. Bennett, M. (2009). Glutamate induces directed chemotaxis of microglia. Eur J Neurosci. 29(6):1108-18.spa
dc.relation.referencesLokensgard, J. Hu, S. Sheng, W. VanOijen, M. Cox, D. Cheeran, M. et al. (2001). Robust expression of TNF-alpha, IL-1beta, RANTES, and IP- 10 by human microglial cells during nonproductive infection with herpes simplex virus. J Neurovirol. 7(3):208-19.spa
dc.relation.referencesMack, C. Vanderlugt-Castaneda, C. Neville, K. Miller, S. (2003). Microglia are activated to become competent antigen presenting and effector cells in the inflammatory environment of the Theiler's virus model of multiple sclerosis. J Neuroimmunol. 144(1-2):68-79.spa
dc.relation.referencesMaezawa, I. & Jin, L. (2010). Rett syndrome microglia damage dendrites and synapses by the elevated release of glutamate. J Neurosci. 30(15): 5346–56.spa
dc.relation.referencesMander, P. Jekabsone, A. Brown, G. (2006). Microglia proliferation is regulated by hydrogen peroxide from NADPH oxidase. J Immunol. 176(2):1046–1052.spa
dc.relation.referencesMarques, C. Cheeran, M. Palmquist, J. Hu, S. Lokensgard, J. (2008). Microglia are the major cellular source of inducible nitric oxide synthase during experimental herpes encephalitis. J Neurovirol. 14(3):229-38.spa
dc.relation.referencesMcCoy, M. & Tansey, M. (2008). TNF signaling inhibition in the CNS: implications for normal brain function and neurodegenerative disease. J Neuroinflammation. 5: 45.spa
dc.relation.referencesMedvedev, A. Sundan, A. Espevik, T. (1994). Involvement of the tumor necrosis factor receptor p75 in mediating cytotoxicity and gene regulating activities. Eur J Immunol. 24(11):2842–49.spa
dc.relation.referencesMehlhorn, G. Hollborn, M. Schliebs, R. (2000). Induction of cytokines in glial cells surrounding cortical beta-amyloid plaques in transgenic Tg2576 mice with Alzheimer pathology. Int J Dev Neurosci. 18(4-5):423-31.spa
dc.relation.referencesMehta, V. Verma, R. Garg, R. Malhotra, H. Sharma, P. Jain, A. (2016). Study of interleukin-6 and interleukin-8 levels in patients with neurological manifestations of dengue. J Postgrad Med. 2016. Epub ahead of print.spa
dc.relation.referencesMelief, J. Koning, N. Schuurman, K. Van De Garde, M. Smolders, J. Hoek, R. et al. (2012). Phenotyping primary human microglia: tight regulation of LPS responsiveness. Glia. 60(10):1506-17.spa
dc.relation.referencesMicheau, O. & Tschopp, J. (2003). Induction of TNF receptor 1-mediated apoptosis via two sequential signaling complexes. Cell. 114(2):181–90.spa
dc.relation.referencesMoussaud S, Draheim H. (2010). A new method to isolate microglia from adult mice and culture them for an extended period of time. J Neurosci Meth. 187: 243–53.spa
dc.relation.referencesMurphy, S. (2000). Production of nitric oxide by glial cells: regulation and potential roles in the CNS. Glia. 29(1): 1–13.spa
dc.relation.referencesMurugan, M. Ling, E. Kaur, C. (2013). Glutamate receptors in microglia. CNS & Neurological Disorded Drug Targets. 12(6):773-84.spa
dc.relation.referencesNakajima, K. & Kohsaka, S. (2001). Microglia: Activation and their significance in the central nervous system. J Biochem. 130(2):169–175.spa
dc.relation.referencesNakamura, Y. Ohmaki, M. Murakami, K. Yoneda, Y. (2003). Involvement of protein kinase C in glutamate release from cultured microglia. Brain Res. 962(1-2):122-8.spa
dc.relation.referencesNimmerjahn, A. Kirchhoff, F. Helmchen, F. (2005). Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. Science. 308(5726):1314–18.spa
dc.relation.referencesOrenstein, J. Meltzer, M. Phipps, T. Gendelman, H. (1988). Cytoplasmic assambly and accumulation of human immunodeficiency virus types 1 and 2 in recombinant human colony-stimulating factor-i-treated human monocytes: an ultrastructural study. J Virol. 62: 2578-86.spa
dc.relation.referencesOrganización mundial de la salud (OMS). (2009). DENGUE: Guías para el diagnóstico, tratamiento, prevención y control.spa
dc.relation.referencesPawate, S. Shen, Q. Fan, F. Bhat, N. (2004). Redox regulation of glial inflammatory response to lipopolysaccharide and interferon gamma. J Neurosci Res. 77(44): 540–51.spa
dc.relation.referencesPennell, N. Hurley, S. Streit, W. (1994). Lectin staining of sheep microglia. Histochemistry. 102(6):483-86.spa
dc.relation.referencesPerry, V. & Holmes, C. (2014). Microglial priming in neurodegenerative disease. Nat Rev Neurol. 10(4): 217–24.spa
dc.relation.referencesPersson, M. Brantefjord, M. Hansson, E. Ronnback, L. (2005) Lipopolysaccharide increases microglial GLT-1 expression and glutamate uptake capacity in vitro by a mechanism dependent on TNF-α. Glia. 51: 111–120.spa
dc.relation.referencesPeudenier, S. Hery, C. Montagnier, L. Tardieu, M. (1991). Human microglial cells: characterization in cerebral tissue and in primary culture, and study of their susceptibility to HIV-1 infection. Ann Neurol. 29(2):152-61.spa
dc.relation.referencesPonomarev, E. Novikova, M. Maresz, K. Shriver, L. Dittel, B. (2005). Development of a culture system that supports adult microglial cell proliferation and maintenance in the resting state. J Immunol Methods. 300(1−2):32–46.spa
dc.relation.referencesPrinz, M. Priller, J. Sisodia, S. Ransohoff, R. (2011). Heterogeneity of CNS myeloid cells and their roles in neurodegeneration. Nat Neurosci. 14(10):1227–35.spa
dc.relation.referencesPrinz, M. & Priller, J. (2014). Microglia and brain macrophages in the molecular age: from origin to neuropsychiatric disease. Natu Rev Neurosci. 15(5): 300-12.spa
dc.relation.referencesProw, N. Irani, D. (2008). The inflammatory cytokine, interleukin-1 beta, mediates loss of astroglial glutamate transport and drives excitotoxic motor neuron injury in the spinal cord during acute vial encephalomyelitis. J Neurochem. 105:1276–86.spa
dc.relation.referencesQin, L. Liu, Y. Wang, T. Wei, S. Block, M. Wilson, B. et al. (2004). NADPH oxidase mediates lipopolysaccharide-induced neurotoxicity and proinflammatory gene expression in activated microglia. J Biol Chem, 279(2):1415-21.spa
dc.relation.referencesRadi, R. Beckman, J. Bush, K. Freeman, B. (1991): Peroxynitrite oxidation of sulfhydryls: the cytotoxic potential of superoxide and nitric oxide. J Biol Chem 266:4244–50.spa
dc.relation.referencesRaivich, G. Bohatschek, M. Kloss, C. Werner, A. Jones, L. Kreutzberg G. (1999). Neuroglial activation repertoire in the injured brain: graded response, molecular mechanisms and cues to physiological function. Brain Res Rev. 30:77–105.spa
dc.relation.referencesRansohoff, R. Perry, V. (2009). Microglial physiology: unique stimuli, specialized responses. Annu Rev Immunol. 27:119–45.spa
dc.relation.referencesRekling, J. (2003). Neuroprotective effects of anticonvulsants in rat hippocampal slice cultures exposed to oxygen/glucose deprivation. Neurosci Lett. 335:167-70.spa
dc.relation.referencesRushil, S. Bhatt, S. Kothari, T, Devanshi G, D’Souza, M. Abhay S. et al. (2015). Novel evidence of microglial immune response in impairment of Dengue infection of CNS. Immunobiol. 220(10):1170-6spa
dc.relation.referencesSahu, R. Verma, R. Jain, A. Garg, R. Singh, M. Malhotra, H. et al. (2014). Neurologic complications in dengue virus infection: a prospective cohort study. Neurology. 83(18):1601-09.spa
dc.relation.referencesSantos, N. Azoubel, A. Lopes, A. Costa, G. Bacellar, A. (2004). Guillain- Barré Syndrome in the course of dengue. Case report Arq NeuroPsiquiatr. 62(1):144-46.spa
dc.relation.referencesSaura, J. (2007). Microglial cells in astroglial cultures: a cautionary note. J neuroinflam. 4: 26-37.spa
dc.relation.referencesShakespear, M. Halili, M. Irvine, K. Fairlie, D. Sweet, M. (2011). Histone deacetylases as regulators of inflammation and immunity. Trends Immunol. 32: 335-43.spa
dc.relation.referencesShen, Y. Li, R. Shiosaki, K. (1997). Inhibition of p75 tumor necrosis fact receptor by antisense oligonucleotides increases hypoxic injury and betaamyloid toxicity in human neuronal cell line. J Biol Chem. 272(6):3550–3553.spa
dc.relation.referencesStreit, W. Morioka, T. Kalehua, A. (1992). MK-801 prevents microglial reaction in rat hippocampus after forebrain ischemia. Neuroreport. 3(2):146148.spa
dc.relation.referencesStreit, W. Walter, S. Pennel, N. (2000). Reactive microgliosis. Prog Neurobiol. 57:563– 581.spa
dc.relation.referencesStreit, W. Hurley, S. McGraw, T. Semple-Rowland, S. (2000). Comparative evaluation of cytokine profiles and reactive gliosis supports a critical role for interleukin-6 in neuron-glia signaling during regeneration. J Neurosci Res. 61:10–20.spa
dc.relation.referencesStreit, W. Xue, Q. (2009). Life and death of microglia. J Neuroimmune Pharmacol. 4(4):371-9.spa
dc.relation.referencesSedgwick J, Schwender S, Imrich H, Dorries R, Butcher G. (1991). Isolation and direct characterisation of resident microglial cells from the normal and inflamed central nervous system. Proc Natl Acad Sci U S A. 88:7438–42spa
dc.relation.referencesSuvannavejh, G. Dal Canto, M. Matis, L. Miller, S. (2000). Fasmediated apoptosis in clinical remissions of relapsing experimental autoinmune encephalomyelitis. J Clin Invest. 105(2):223-231.spa
dc.relation.referencesTakeuchi, H. Jin, S. Wang, J. Zhang, G. Kawanokuchi, J.Kuno, R. et al. (2006). Tumor necrosis factor-� induces neurotoxicity via glutamate release from hemichannels of activated microglia in an autfocrine manner. J Biol Chem. 281(30):21362-8.spa
dc.relation.referencesTansey, M. Frank-Cannon, T. McCoy, M. Lee, J. Martinez, T. McAlpine, F., et al. (2008). Neuroinflammation in Parkinson’s disease: is there sufficient evidence for mechanism-based interventional therapy? Front Biosci.13: 709–17.spa
dc.relation.referencesTartaglia, L. Goeddel, D. (1992). Two TNF receptors. Immunol Today. 13(5):151–153.spa
dc.relation.referencesTaylor, D. Jones, F. Kubota, E. Pocock, J. (2005). Stimulation of microglial metabotropic glutamate receptor mGlu2 triggers tumor necrosis factor α induced neurotoxicity in concert with microglial-derived Fas ligand. J Neurosci. 25(11): 2952- 2964.spa
dc.relation.referencesThongtan, T. Cheepsunthorn, P. Chaiworakul, V, Rattanarungsan, C. Wikan, N. Smith, D. (2010). Highly permissive infection of microglial cells by Japanese encephalitis virus: a possible role as a viral reservoir. Microbes Infect. 12(1): 37-47.spa
dc.relation.referencesThorburn, A. (2004). Death receptor-induced cell killing. Cell Signal. 16(2):139–144.spa
dc.relation.referencesTolosa, L. Caraballo-Miralles, V. Olmos, G. Llado, J. (2011). TNF- α potentiates glutamate -induced spinal cord motoneuron death via NF- kB. Mol Cell Neurosci. 46:176–86.spa
dc.relation.referencesTsung-Ting, T. Chia-Ling, Ch. Yee-Shin, L. Chih-Peng, Ch. ChengChieh, T. et al. (2016). Microglia retard dengue virus-induced acute viral encephalitis. Sci Rep. 6:27670.spa
dc.relation.referencesTurchan-Cholewo, J. Dimayuga, V. Gupta, S. Gorospe, R. Keller, J. Bruce- Keller, A. (2009). NADPH oxidase drives cytokine and neurotoxin release from microglia and macrophages in response to HIV-Tat. Antioxid Redox Signal. 11(2):193-204.spa
dc.relation.referencesValko, M. Leibfritz, D. Moncol, J. Cronin, M. Mazur, M. Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 39(1):44-84.spa
dc.relation.referencesVelandia, M. & Castellanos, J. (2011). Dengue virus: structure and viral cycle.Infectio. 15(1): 33-43.spa
dc.relation.referencesVelandia, M. & Castellanos, J. (2012). Flavivirus Neurotropism, Neuroinvasion, Neurovirulence and Neurosusceptibility Clues to Understanding Flavivirus- and Dengue-Induced Encephalitis. En: García ML, Romanowski V, editores. Viral Genomes– Molecular Structure, Diversity, Gene Expression Mechanisms and Host- Virus Interactions. Croacia: InTech: 219-40.spa
dc.relation.referencesVelandia, M. Acosta, O. Castellanos, J. (2012). In vivo infection by a neuroinvasive neurovirulent dengue virus J Neurovirol. 18:374–87.spa
dc.relation.referencesVerma, R. Sahu, R. Holla, V. (2014). Neurological manifestations of dengue infection: a review. J Neurol Sci. 346(1-2):26-34.spa
dc.relation.referencesVilcek, J. 1998. The cytokines: an overview. In: Thomson A, editor. The cytokine handbook. San Diego: Academic Press. 1–20.spa
dc.relation.referencesWalker, D. Whetzel, A. Lue, L. (2015). Expression of suppressor of cytokine signaling genes in human elderly and Alzheimer's disease brains and human microglia. Neuroscience. 302: 121-37.spa
dc.relation.referencesWinter, J. Lehmann, T. Krauss, S. Trockenbacher, A. Kijas, Z. Foerster, J. et al. (2004). Regulation of the MID1 protein function is fine-tuned by a complex pattern of alternative splicing. Hum Genet. 114(6):54152.spa
dc.relation.referencesXuan, A. Pan, X. Wei, P. Ji, W. Zhang, W. Liu, J. et al. (2014). Valproic Acid Alleviates Memory Deficits and Attenuates Amyloid-β Deposition in Transgenic Mouse Model of Alzheimer's Disease. Mol Neurobiol. 51(1):30012.spa
dc.relation.referencesYang, C. Lee, H. Lee, J. Kim, J. Lee, S. Shin, D. et al. (2007). Reactive oxygen species and p47phox activation are essential for the Mycobacterium tuberculosis-induced pro-inflammatory response in murine microglia. J Neuroinflam. 4:27.spa
dc.relation.referencesYe, L. Huang, Y.Zhao, L. Li, Y. Sun, L. Zhou, Y. et al. (2013). IL-1beta and TNF-α induce neurotoxicity through glutamate production: a potential role for neuronal glutaminase. J Neurochem. 125(6): 897–908.spa
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons2016
dc.rights.localAcceso abiertospa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectCélulas de la microglíaspa
dc.subjectNeuropatogeniaspa
dc.subjectDENVspa
dc.subjectVirus neuroadapatadospa
dc.subjectActivación celularspa
dc.subject.decsNeurotoxinasspa
dc.subject.decsTécnicas in vitrospa
dc.subject.decsVirus del denguespa
dc.subject.nlmW 50
dc.titleEvaluación in vitro de los agentes neurotóxicos liberados por las células de la microglía, infectadas con el virus dengue neuroadaptado (D4MB-6)spa
dc.title.translatedIn vitro assessment of the neurotoxic agents produced by infected microglial cells by a neuroadapted strain of Dengue Virus-D4MB-6spa
dc.type.coarhttps://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttps://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersion
dc.type.localTesis/Trabajo de grado - Monografía - Maestríaspa

Archivos

Bloque original
Mostrando 1 - 2 de 2
Miniatura
Nombre:
Beltrán_Zúñiga_Edgar_Orlando_2016.pdf
Tamaño:
8.51 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
No hay miniatura disponible
Nombre:
Beltrán_Zúñiga_Edgar_Orlando_2016_Carta_autorizacion.pdf
Tamaño:
498.21 KB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

Maestría en Ciencias Básicas Biomédicas