Semicontinuous system for the production of recombinant mCherry protein in Chlamydomonas reinhardtii

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dc.contributor.authorDiaz Arias, Cesar Andres
dc.contributor.authorMatsudo, Marcelo Chuei
dc.contributor.authorFerreira-Camargo, Livia Seno
dc.contributor.authorDutra Molino, João Vitor
dc.contributor.authorMayfield, Stephen Patrick
dc.contributor.authorMonteiro de Carvalho, João Carlos
dc.date.accessioned2021-05-21T16:26:31Z
dc.date.available2021-05-21T16:26:31Z
dc.date.issued2021-11-09
dc.description.abstractenglishBiotechnology advances have allowed bacteria, yeasts, plants, mammalian and insect cells to function as heterologous protein expression systems. Recently, microalgae have gained attention as an innovative platform for recombinant protein production, due to low culture media cost, compared to traditional systems, as well as the fact that microalgae such as Chlamydomonas reinhardtii are considered safe (GRAS) by the Food and Drug Administration (FDA). Previous studies showed that recombinant protein production in traditional platforms by semicontinuous process increased biomass and bio product productivity, when compared to batch process. As there is a lack of studies on semicontinuous process for recombinant protein production in microalgae, the production of recombinant mCherry fluorescent protein was evaluated by semicontinuous cultivation of Chlamydomonas reinhardtii in bubble column photobioreactor. This semicontinuous cultivation process was evaluated in the following conditions: 20%, 40%, and 60% culture portion withdrawal. The highest culture withdrawal percentage (60%) provided the best results, as an up to 161% increase in mCherry productivity (454.5 RFU h−1 – Relative Fluorescence Unit h−1), in comparison to batch cultivation (174.0 RFU h−1) of the same strain. All cultivations were carried out for 13 days, at pH 7, temperature 25°C and, by semicontinuous process, two culture withdrawals were taken during the cultivations. Throughout the production cycles, it was possible to obtain biomass concentration up to 1.36 g L−1.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1002/btpr.3101
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1520-6033
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/5885
dc.language.isoeng
dc.publisherWiley-Blackwellspa
dc.publisher.journalBiotechnology Progressspa
dc.relation.ispartofseriesBiotechnology Progress, 1520-6033, Vol. 37, Nro. 2, 2021spa
dc.relation.urihttps://aiche.onlinelibrary.wiley.com/doi/abs/10.1002/btpr.3101
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.localAcceso abiertospa
dc.subject.keywordsBiotechnologyspa
dc.subject.keywordsBubble column photobioreactorspa
dc.subject.keywordsHeterologous proteinspa
dc.subject.keywordsMicroalgae cultivationspa
dc.titleSemicontinuous system for the production of recombinant mCherry protein in Chlamydomonas reinhardtiispa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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