Detection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin America

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dc.contributor.authorCastro, Betsy Esperanza
dc.contributor.authorBerrío, Maritza
dc.contributor.authorVargas, Monica L.
dc.contributor.authorCarvajal Ortiz, Lina Paola
dc.contributor.authorMillan, Lina V.
dc.contributor.authorRios, Rafael
dc.contributor.authorHernandez, Angie K.
dc.contributor.authorRincon Núñez, Sandra
dc.contributor.authorCubides, Paola
dc.contributor.authorForero, Erika
dc.contributor.authorDinh, An Q
dc.contributor.authorSeas, Carlos
dc.contributor.authorMunita, Jose M.
dc.contributor.authorArias, César A.
dc.contributor.authorReyes, Jinnethe
dc.contributor.authorDiaz, Lorena
dc.contributor.orcidRincon Núñez, Sandra [0000-0002-8482-4554]
dc.contributor.orcidCarvajal Ortiz, Lina Paola [0000-0001-8301-8836]
dc.date.accessioned2020-09-01T21:21:47Z
dc.date.available2020-09-01T21:21:47Z
dc.date.issued2020
dc.description.abstractenglishBackground:Vancomycin is a common first-line option for MRSA infections. The heterogeneous vancomycin-intermediateStaphylococcus aureus(hVISA) phenotype is associated with therapeutic failure. However, hVISAisolates are usually reported as vancomycin susceptible by routine susceptibility testing procedures.Objectives:To detect and characterize the hVISA phenotype in MRSA isolates causing infections in nine LatinAmerican countries.Methods:We evaluated a total of 1189 vancomycin-susceptible MRSA isolates recovered during 2006–08 and2011–14. After an initial screening of hVISA using glycopeptide-supplemented agar strategies, the detection ofhVISA was performed by Etest (GRD) and Macro-method (MET). Isolates deemed to be hVISA were subjectedto population analysis profile/AUC (PAP/AUC) and WGS for further characterization. Finally, we interrogatedalterations in predicted proteins associated with the development of the VISA phenotype in both hVISA andvancomycin-susceptibleS. aureus(VSSA) genomes.Results:A total of 39 MRSA isolates (3.3%) were classified as hVISA (1.4% and 5.6% in MRSA recovered from2006–08 and 2011–14, respectively). Most of the hVISA strains (95%) belonged to clonal complex (CC) 5. Only6/39 hVISA isolates were categorized as hVISA by PAP/AUC, with 6 other isolates close (0.87–0.89) to the cut-off(0.9). The majority of the 39 hVISA isolates exhibited the Leu-14!Ile (90%) and VraT Glu-156!Gly (90%) aminoacid substitutions in WalK. Additionally, we identified 10 substitutions present only in hVISA isolates, involvingWalK, VraS, RpoB and RpoC proteins.Conclusions:The hVISA phenotype exhibits low frequency in Latin America. Amino acid substitutions in proteinsinvolved in cell envelope homeostasis and RNA synthesis were commonly identified. Our results suggest thatEtest-based methods are an important alternative for the detection of hVISA clinical isolates.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1093/jac/dkaa221
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1460-2091
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlhttps://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/3909
dc.language.isoeng
dc.publisherOxford University Pressspa
dc.publisher.journalJournal of antimicrobial chemotherapyspa
dc.relation.ispartofseriesJournal of antimicrobial chemotherapy, 1460-2091, Vol. 74, Nro. 9, 2020, p. 2424-2431spa
dc.relation.urihttps://academic.oup.com/jac/article-abstract/75/9/2424/5860393
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2020-04-30
dc.rights.localAcceso abiertospa
dc.subject.keywordsPhenotypespa
dc.subject.keywordsVancomycinspa
dc.subject.keywordsGaitspa
dc.subject.keywordsHeterogeneityspa
dc.titleDetection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin Americaspa
dc.title.translatedDetection of heterogeneous vancomycin intermediate resistance in MRSA isolates from Latin Americaspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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