Study of interferon-β antiviral activity against Herpes simplex virus type 1 in neuron-enriched trigeminal ganglia cultures

Vista sencilla de ítem
dc.contributor.authorCalle, Ana
dc.contributor.authorPrada-Arismendy, Jeanette
dc.contributor.authorCastellanos, Jaime
dc.contributor.orcidCastellanos, Jaime [0000-0003-1596-8383]
dc.date.accessioned2020-07-14T19:51:46Z
dc.date.available2020-07-14T19:51:46Z
dc.date.issued2014
dc.description.abstractenglishHerpes simplex virus type 1 (HSV-1) causes a lytic infection in epithelial cells before being captured and moved via retrograde axonal transport to the nuclei of the sensory neurons of the trigeminal ganglion or dorsal root, where it establishes a latent infection. HSV-1 infection induces an antiviral response through the production of Beta Interferon (IFN-β) in infected trigeminal ganglia. The aim of this work was to characterize the response induced by IFN-β in neuron-enriched trigeminal ganglia primary cultures infected with HSV-1. An antiviral effect of IFN-β in these cultures was observed, including reduced viral production and increased cell survival. In contrast, viral infection significantly decreased both double stranded RNA dependent protein kinase (Pkr) transcription and Jak-1 and Stat-1 phosphorylation, suggesting a possible HSV-1 immune evasion mechanism in trigeminal cells. Additionally, HSV-1 infection upregulated Suppressor of Cytokine Signaling-3 (Socs3) mRNA; upregulation of socs3 was inhibited in IFN-β treated cultures. HSV-1 infection increased the number of Socs3 positive cells and modified the intracellular distribution of Socs3 protein, in infected cells. This neuron-enriched trigeminal ganglia culture model could be used to elucidate the HSV-1 viral cycle in sensory neurons and to study cellular antiviral responses and possible viral evasion mechanisms that underlie the choice between viral replication and latency.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.virusres.2013.12.022
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1872-7492
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlhttps://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/3488
dc.language.isoeng
dc.publisherElsevierspa
dc.publisher.journalVirus Researchspa
dc.relation.ispartofseriesVirus Research, 1872-7492, Vol. 180, 2014 p. 49-58spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S0168170213004516
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2014
dc.rights.localAcceso abiertospa
dc.subject.keywordsHSV-1spa
dc.subject.keywordsInterferon-βspa
dc.subject.keywordsTrigeminal ganglionspa
dc.subject.keywordsSocs3spa
dc.subject.keywordsJak-Statspa
dc.titleStudy of interferon-β antiviral activity against Herpes simplex virus type 1 in neuron-enriched trigeminal ganglia culturesspa
dc.title.translatedStudy of interferon-β antiviral activity against Herpes simplex virus type 1 in neuron-enriched trigeminal ganglia culturesspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Low_Calle_Ana_Maria_2014.pdf
Tamaño:
2.47 MB
Formato:
Adobe Portable Document Format
Descripción:
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

Artículos