Determinación in vitro de la participación de la proteína hipotética SAUSA300_0063 en la activación del operón arc presente en el elemento móvil para el catabolismo de la arginina (ACME) en el clon pandémico USA300

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dc.contributor.advisorEscobar-Pérez, Javier
dc.contributor.advisorMárquez Ortiz, Ricaurte Alejandro
dc.contributor.authorCorredor Rozo, Zayda Lorena
dc.contributor.orcidEscobar-Pérez, Javier [0000-0002-0432-6978]
dc.date.accessioned2021-02-16T19:34:16Z
dc.date.available2021-02-16T19:34:16Z
dc.date.issued2021
dc.description.abstractEn las últimas dos décadas se ha reportado la emergencia y rápida diseminación del Staphylococcus aureus resistente a meticilina clon USA300 con una amplia distribución y patogenicidad, causando al ser humano infecciones no solo en el hospital sino en la comunidad. El elemento genético móvil para el catabolismo de la arginina (ACME) fue identificado exclusivamente en este clon y dentro de este elemento se encuentra un operón arc que tiene la misma función catabólica de ACME suministrando ATP en condiciones anaerobias y que en ambientes ácidos aumenta el pH del medio favoreciendo la capacidad para colonizar. Estudios previos en nuestro laboratorio mostraron que una sobreexpresión del operón arcACME en el clon USA300 es dada posiblemente por el gen sausa300_0063 inserto dentro de este operón que codifica para un regulador transcripcional. Determinar la participación de la proteína hipotética SAUSA300_0063 en la activación del operón arc presente en ACME en el clon pandémico USA300. Mediante el sistema de expresión pET303/CT-His en BL21 (DE3) se produjo la proteína recombinante SAUSA300_0063 del operón arcACME la cual se purificó mediante electroelución. Se determinó la unión in vitro de la proteína recombinante SAUSA300_0063 a la secuencia promotora del operón arcACME mediante ensayos de retardamiento en gel y por PCR en tiempo real se analizó la transcripción relativa de los genes sausa300_0063, arcCACME, arcCcons y arcR en condiciones de anaerobiosis en presencia o ausencia de arginina. La evidencia experimental mostró que la proteína SAUSA300_0063 establece su unión en el promotor del operón arcACME sugiriendo una aproximación acerca de su función como regulador transcripcional perteneciente a la familia de proteínas CRP/FNR. Adicionalmente se observó una relación directa en el aumento de la transcripción de los genes sausa300_0063 y arcC del operón arcACME indicando la posible participación de la proteína SAUSA300_0063 en la autoactivación del operón arcACME, dada a esta nueva estructura del operón facilitando su regulación. La proteína SAUSA300_0063 participa en la activación del operón arcACME a través de la unión a su región promotora y esta activación es mayor que la encontrada en el operón arc constitutivo. Estos resultados sugieren que el cambio estructural encontrado en el operón arcACME facilita la participación de la proteína SAUSA300_0063 en la auto-activación de este operón ya que dada a esta nueva estructura se podría regular en modo cis considerándose un sistema altamente ventajoso.spa
dc.description.abstractenglishIn the last two decades has reported the emergence and rapid spread of methicillinresistant Staphylococcus aureus USA300 clone with a wide distribution and pathogenicity, causing human infections not only in the hospital but in the community. The mobile genetic element for catabolism of arginine (ACME) was identified only in this clone and inside this element is one arc operon having the same catabolic function ACME providing ATP under anaerobic conditions and in acidic environments increases pH medium favoring the ability to colonize. Previous studies in our laboratory showed that overexpression arcACME operon in clone USA300 is possibly given by the insert sausa300_0063 within this operon gene encoding a transcriptional regulator. Determine the participation SAUSA300_0063 hypothetical protein in the activation of arcACME operon in the USA300 clone pandemic. Using the system pET303 / CT-His expression in BL21 (DE3) recombinant protein produced SAUSA300_0063 arcACME operon which was purified by electroelution. In vitro binding of recombinant protein SAUSA300_0063 to the promoter sequence of the arcACME operon determined by assays retarding gel and real-time PCR the relative transcription sausa300_0063, arcCACME, arcCcons and arcR genes it was analyzed in anaerobiosis in presence or absence of arginine. Experimental evidence showed that establishes its binding protein SAUSA300_0063 in arcACME operon promoter suggesting an approach about its function as a transcriptional regulator belonging to the family of proteins CRP / FNR. Additionally a direct relationship in the increased transcription of sausa300_0063 genes and arcC operon arcACME indicating the possible participation SAUSA300_0063 protein self-activation arcACME operon given to this new structure operon facilitating regulation. SAUSA300_0063 protein participes in the activation arcACME operon across binding to its promoter region and this activation is greater than that found in the constituent arc operon. These results suggest that the structural change in the arcACME operon found facilitates participation SAUSA300_0063 self-protein in the activation of this operon and given to this new structure could be regulated in cis mode considered a highly advantageous system.eng
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias Básicas Biomédicasspa
dc.format.mimetypeapplication/pdf
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/5348
dc.language.isospa
dc.publisher.facultyFacultad de Medicinaspa
dc.publisher.grantorUniversidad El Bosquespa
dc.publisher.programMaestría en Ciencias Básicas Biomédicasspa
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dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons2015-08
dc.rights.localAcceso abiertospa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectClon USA300spa
dc.subjectACMEspa
dc.subjectOperón arcspa
dc.subjectProteína hipotética SAUSA300_0063spa
dc.subjectActivador transcripcionalspa
dc.subject.decsAnálisis de secuencia de proteínaspa
dc.subject.decsMetabolismospa
dc.subject.decsTécnicas in vitrospa
dc.subject.keywordsClone USA300spa
dc.subject.keywordsACMEspa
dc.subject.keywordsOperon arcspa
dc.subject.keywordsSAUSA300_0063spa
dc.subject.keywordsHypothetical proteinspa
dc.subject.keywordsTranscriptional activatorspa
dc.subject.nlmW 50
dc.titleDeterminación in vitro de la participación de la proteína hipotética SAUSA300_0063 en la activación del operón arc presente en el elemento móvil para el catabolismo de la arginina (ACME) en el clon pandémico USA300spa
dc.title.translatedDetermination in vitro protein participation in the activation hypothetical Sausa300_0063 operon ARC present in the mobile element for catabolism of arginine (ACME) in clone Usa300 pandemicspa
dc.type.coarhttps://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttps://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersion
dc.type.localTesis/Trabajo de grado - Monografía - Maestríaspa

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Maestría en Ciencias Básicas Biomédicas