Genomic and epidemiological evidence of a dominant panton-valentine leucocidin-positive methicillin resistant staphylococcus aureus lineage in Sri Lanka and presence among isolates from the United Kingdom and Australia

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dc.contributor.authorMcTavish, Sharla M.
dc.contributor.authorSnow, Sarah J.
dc.contributor.authorCook, Ellie C.
dc.contributor.authorPichon, Bruno
dc.contributor.authorColeman, Sarah
dc.contributor.authorCoombs, Geoffrey W.
dc.contributor.authorPang, Stanley
dc.contributor.authorArias, César A.
dc.contributor.authorDíaz, Lorena
dc.contributor.authorBoldock, Emma
dc.contributor.authorDavies, Steve
dc.contributor.authorUdukala, Mangala
dc.contributor.authorKearns, Angela Marie
dc.contributor.authorSiribaddana, Sisira
dc.contributor.authorDe Silva, Thushan I.
dc.date.accessioned2019-11-18T17:38:13Z
dc.date.available2019-11-18T17:38:13Z
dc.date.issued2019
dc.description.abstractenglishObjective: To undertake the first detailed genomic analysis of methicillin-resistant Staphylococcus aureus (MRSA) isolated in Sri Lanka. Methods: A prospective observational study was performed on 94 MRSA isolates collected over a 4 months period from the Anuradhapura Teaching Hospital, Sri Lanka. Screening for mecA, mecC, and the Panton-Valentine leucocidin (PVL)-associated lukS-PV/lukF-PV genes and molecular characterization by spa typing was undertaken. Whole genome sequencing (WGS) and phylogenetic analysis was performed on selected multilocus sequence type (MLST) clonal complex 5 (CC5) isolates from Sri Lanka, England, Australia, and Argentina. Results: All 94 MRSA harbored the mecA gene. Nineteen spa types belonging to nine MLST clonal complexes were identified. Where origin of the sample was recorded, most isolates were from skin and soft tissue infections (70/91; 76.9%), with fewer causing bacteremia (16/91; 17.6%), empyema (3/91; 3.3%) and osteomyelitis (2/91; 2.2%). Sixty two (65.9%) isolates were PVL positive with the majority (56 isolates; 90.3%) belonging to a dominant CC5 lineage. This lineage, PVL-positive ST5-MRSA-IVc, was associated with both community and hospital-onset infections. Based on WGS, representative PVL-positive ST5-MRSA-IVc isolates from Sri Lanka, England and Australia formed a single phylogenetic clade, suggesting wide geographical circulation. Conclusions: We present the most detailed genomic analysis of MRSA isolated in Sri Lanka to date. The analysis identified a PVL-positive ST5-MRSA-IVc that is prevalent among MRSA causing clinical infections in Sri Lanka. Furthermore, this clone was also found among isolates from the United Kingdom and Australia.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://dx.doi.org/10.3389%2Ffcimb.2019.00123
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn2235-2988
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/1853
dc.language.isoeng
dc.publisherFrontiers Mediaspa
dc.publisher.journalFrontiers in Cellular and Infection Microbiologyspa
dc.relation.ispartofseriesFrontiers in Cellular and Infection Microbiology, 2235-2988, Vol. 9, Nro. 123, 2019spa
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fcimb.2019.00123/full
dc.rightsAttribution 4.0 International*
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf216
dc.rights.creativecommons2019
dc.rights.localAcceso abiertospa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.decsStaphylococcus aureus resistente a meticilinaspa
dc.subject.decsLeucocidinasspa
dc.subject.decsCódigo de barras del ADN taxonómicospa
dc.subject.keywordsWhole genome sequencingspa
dc.subject.keywordsCC5spa
dc.subject.keywordsSri Lankaspa
dc.titleGenomic and epidemiological evidence of a dominant panton-valentine leucocidin-positive methicillin resistant staphylococcus aureus lineage in Sri Lanka and presence among isolates from the United Kingdom and Australiaspa
dc.typearticlespa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localartículospa

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