Studying neurotrophin antiviral effect on rabies-infected dorsal root ganglia cultures

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dc.contributor.authorCastellanos, Jaime
dc.contributor.authorMartínez-Gutiérrez, Marlén
dc.contributor.authorHurtado, Hernán
dc.contributor.authorKassis, Raïd
dc.contributor.authorBourhy, Hervé
dc.contributor.authorAcosta, Orlando
dc.contributor.authorLafon, Monique
dc.date.accessioned2021-02-23T16:13:38Z
dc.date.available2021-02-23T16:13:38Z
dc.date.issued2005
dc.description.abstractenglishNeurotrophin (NT)-induced modulation of rabies virus adsorption, transcription, and replication were analyzed in adult mouse dorsal root ganglia cultures. Different types of nerve growth factor and NT-3 treatment were tested before infection (pretreatment), during infection (transtreatment) and after withdrawing the viral inoculum (post-treatment). NT pretreatment for 4 days prior to infection produced a significant increase in the quantity of virus adsorbed into cultures and a concomitant increase in genomic viral RNA as measured by real time polymerase chain reaction (PCR). NT pretreatment triggered increased expression of two rabies virus receptors (NCAM and p75NTR); however, no increase in rabies virus transcription and expression could be observed. By contrast, NT treatment during and after infection (trans- and post-treatment) induced a strong decrease in the quantity of viral nucleoprotein genomic and messenger nucleoprotein RNAs. These findings suggested that NT had an intrinsic inhibitory effect on rabies virus infection, which was not counterbalanced by NTs’ rabies virus receptor—enhancing property and viral uptake. Adult mouse dorsal root ganglion cultures can be regarded as being a useful model for detecting therapeutic targets and evaluating experimental antiviral drugs.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1080/13550280500187252
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1538-2443
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlhttps://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/5420
dc.language.isoeng
dc.publisherSpringer Naturespa
dc.publisher.journalJournal of neuroVirologyspa
dc.relation.ispartofseriesJournal of neuroVirology, 1538-2443, Vol. 11, Nor. 4, 2005, p. 403-410spa
dc.relation.urihttps://link.springer.com/article/10.1080/13550280500187252
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2005-07
dc.rights.localAcceso abiertospa
dc.subject.keywordsAntiviral effectspa
dc.subject.keywordsNeurotrophinsspa
dc.subject.keywordsRabiesspa
dc.subject.keywordsReal time PCRspa
dc.subject.keywordsSensory neuronspa
dc.titleStudying neurotrophin antiviral effect on rabies-infected dorsal root ganglia culturesspa
dc.title.translatedStudying neurotrophin antiviral effect on rabies-infected dorsal root ganglia culturesspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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