Preadministration of yerba mate (Ilex paraguariensis) helps functional activity and morphology maintenance of MC3T3-E1 osteoblastic cells after in vitro exposition to hydrogen peroxide

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dc.contributor.authorCeverino, Gabrielli Collasanto
dc.contributor.authorVargas Sanchez, Paula Katherine
dc.contributor.authorFernandes, Roger Rodrigo
dc.contributor.authorAlves, Guilherme Alvarenga
dc.contributor.authorde Santis, José Bernardo
dc.contributor.authorTavares, Milla Sprone
dc.contributor.authorSiéssere, Selma
dc.contributor.authorBombonato-Prado, Karina Fittipaldi
dc.contributor.orcidVargas Sanchez, Paula Katherine [0000-0002-6554-4741]
dc.date.accessioned2021-04-13T22:38:44Z
dc.date.available2021-04-13T22:38:44Z
dc.date.issued2021-01-17
dc.description.abstractenglishNatural substances with antioxidant effects may benefit prevention and treatment of people with or prone to bone diseases after menopause, such as osteoporosis. This study aimed to evaluate the in vitro effect of preadministration of yerba mate extract (YM) in the metabolism of MC3T3-E1 osteoblasts exposed to hydrogen peroxide (H2O2). The cells (MC3T3-E1) were cultured in 24-well plates with the concentration of 1 μg/mL yerba mate extract dissolved in culture medium throughout the culture period. Four hours before each experiment, 400 μmol/L H2O2 was added per well to simulate oxidative stress. There were evaluated cell adhesion and proliferation, in situ detection of alkaline phosphatase (ALP), mineralized nodules, and immunolocalization of osteocalcin (OCN), bone sialoprotein (BSP) and alkaline phosphatase (ALP) proteins. The results showed that YM preadministration to H2O2 exposition significatively increased cell adhesion after 3 days as well as proliferation and in situ ALP detection after 10 and 7 days respectively, when compared to H2O2 group. Besides, staining of OCN and BSP proteins was less intense and scattered in poor spread cells with cytoskeletal changes in H2O2 group when compared to control and YM H2O2 group. ALP staining was restrained to intracellular regions and similar in all experimental groups. Our results suggest that preadministration of yerba mate extract may prevent deleterious effects in the morphology and functional activity of osteoblasts exposed to H2O2, which could enable the maintenance of extracellular matrix in the presence of oxidative stress.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1007/s11033-020-06096-w
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1573-4978
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/5770
dc.language.isoeng
dc.publisherSpringer Naturespa
dc.publisher.journalMolecular biology reportsspa
dc.relation.ispartofseriesMolecular biology reports, 1573-4978, Vol. 48, Nro. 1, 2021, p. 13-20spa
dc.relation.urihttps://link.springer.com/article/10.1007%2Fs11033-020-06096-w
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.localAcceso abiertospa
dc.subject.keywordsOsteoblastsspa
dc.subject.keywordsYerba mate extractspa
dc.subject.keywordsHydrogen peroxidespa
dc.titlePreadministration of yerba mate (Ilex paraguariensis) helps functional activity and morphology maintenance of MC3T3-E1 osteoblastic cells after in vitro exposition to hydrogen peroxidespa
dc.title.translatedPreadministration of yerba mate (Ilex paraguariensis) helps functional activity and morphology maintenance of MC3T3-E1 osteoblastic cells after in vitro exposition to hydrogen peroxidespa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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