Effect of vitamins D and e on the Proliferation, viability, and differentiation of human dental pulp stem cells: an in vitro study

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dc.contributor.authorVargas Escobar, Lina María
dc.contributor.authorBendahan Álvarez, Zita Carolina
dc.contributor.authorBayona, Andrea
dc.contributor.authorCastellanos, Jaime
dc.contributor.authorGonzález Carrera, María Clara
dc.contributor.orcidCastellanos, Jaime [0000-0003-1596-8383]
dc.contributor.orcidGonzález Carrera, María Clara [0000-0003-4572-1453]
dc.contributor.orcidVargas Escobar, Lina María [0000-0003-0052-5467]
dc.contributor.orcidBendahan Álvarez, Zita Carolina [0000-0002-0661-0251]
dc.date.accessioned2020-07-27T23:52:00Z
dc.date.available2020-07-27T23:52:00Z
dc.date.issued2020
dc.description.abstractenglishntroduction. The aim of the present study was to determine the effects of vitamins D and E on the proliferation, morphology, and differentiation of human dental pulp stem cells (hDPSCs). Methods. In this in vitro experimental study, hDPSCs were isolated, characterized, and treated with vitamins D and E, individually and in combination, utilizing different doses and treatment periods. Changes in morphology and cell proliferation were evaluated using light microscopy and the resazurin assay, respectively. Osteoblast differentiation was evaluated with alizarin red S staining and expression of RUNX2, Osterix, and Osteocalcin genes using real-time RT-PCR. Results. Compared with untreated cells, the number of cells significantly reduced following treatment with vitamin D (49%), vitamin E (35%), and vitamins D + E (61%) after 144 h. Compared with cell cultures treated with individual vitamins, cells treated with vitamins D + E demonstrated decreased cell confluence, with more extensive and flatter cytoplasm that initiated the formation of a significantly large number of calcified nodules after 7 days of treatment. After 14 days, treatment with vitamins D, E, and D + E increased the transcription of RUNX2, Osterix, and Osteocalcin genes. Conclusions. Vitamins D and E induced osteoblastic differentiation of hDPSCs, as evidenced by the decrease in cell proliferation, morphological changes, and the formation of calcified nodules, increasing the expression of differentiation genes. Concurrent treatment with vitamins D + E induces a synergistic effect in differentiation toward an osteoblastic lineage.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1155/2020/8860840
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1687-8736
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlhttps://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/3591
dc.language.isoeng
dc.publisherHindawispa
dc.publisher.journalInternational journal of dentistryspa
dc.relation.ispartofseriesInternational journal of dentistry, 1687-8736, Vol. 2020, 2020spa
dc.relation.urihttps://www.hindawi.com/journals/ijd/2020/8860840/
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2020-07-01
dc.rights.localAcceso abiertospa
dc.subject.decsVitamina Dspa
dc.subject.decsVitamina Espa
dc.subject.decsPulpa dentalspa
dc.titleEffect of vitamins D and e on the Proliferation, viability, and differentiation of human dental pulp stem cells: an in vitro studyspa
dc.title.translatedEffect of vitamins D and e on the Proliferation, viability, and differentiation of human dental pulp stem cells: an in vitro studyspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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