Detection of specific periodontal microorganisms from bacteraemia samples after periodontal therapy using molecular-based diagnostics

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dc.contributor.authorCastillo, Diana Marcela
dc.contributor.authorSánchez‐Beltrán, María Carmen
dc.contributor.authorCastellanos, Jaime
dc.contributor.authorSanz, Ignacio
dc.contributor.authorMayorga‐Fayad, Isabel
dc.contributor.authorSanz, Mariano
dc.contributor.authorLafaurie, Gloria Ines
dc.contributor.orcidCastellanos, Jaime [0000-0003-1596-8383]
dc.contributor.orcidLafaurie, Gloria Ines [0000-0003-3986-0625]
dc.date.accessioned2020-08-31T11:05:55Z
dc.date.available2020-08-31T11:05:55Z
dc.date.issued2011
dc.description.abstractenglishThe aim of this study was to assess the presence of subgingival pathogens in peripheral blood samples from periodontitis patients before and after scaling and root planing (Sc/RP) using nested polymerase chain reaction (nested PCR). Peripheral blood samples were obtained from 42 patients with severe generalized chronic or aggressive periodontitis. In each patient, four samples of peripheral blood were drawn at different times: immediately before the Sc/RP procedure; immediately after Sc/RP; 15 and 30 min. post‐Sc/RP. Blood samples were analysed for bacteraemia with anaerobic culturing and nested PCR, using universal bacterial primers that target the 16S‐rRNA gene of most bacteria, subsequently re‐amplified with specific primers to Porphyromonas gingivalis , Aggregatibacter actinomycetemcomitans , Tannerella forsythia , Eikenella corrodens , Campylobacter rectus and Prevotella intermedia , using a modified phenol–chloroform method for DNA extraction. Presence of specific periodontal pathogens in peripheral blood after treatment was detected in 54.8% of the patients, in 47.6% with anaerobic culturing and in 19% with nested PCR. In 16.6%, the periodontal pathogens were detected before Sc/RP. P. gingivalis and A. actynomicetemcomitans were the pathogens most frequently detected in the bloodstream before and after Sc/RP. Nested PCR demonstrated the presence of DNA from periodontal pathogens in blood samples in severe periodontitis patients before, during and after periodontal therapy. The use of these molecular‐based techniques may improve the accuracy from the results obtained by haemoculture.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1111/j.1600-051X.2011.01717.x
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn0303-6979
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlhttps://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/3893
dc.language.isoeng
dc.publisherWileyspa
dc.publisher.journalJournal of Clinical Periodontologyspa
dc.relation.ispartofseriesJournal of Clinical Periodontology, 0303-6979, Vol. 38, No. 5, 2011 p. 418-427spa
dc.relation.urihttps://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-051X.2011.01717.x
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2011-03
dc.rights.localAcceso abiertospa
dc.subject.decsEnfermedades periodontalesspa
dc.subject.decsBacilos gramnegativos anaerobios rectos, curvos y espiralesspa
dc.subject.decsEndocarditis bacterianaspa
dc.subject.keywordsAggregatibacter actinomycetemcomitansspa
dc.subject.keywordsBacteraemiaspa
dc.subject.keywordsCardiovascular diseasespa
dc.subject.keywordsInfective endocarditisspa
dc.subject.keywordsNested PCRspa
dc.subject.keywordsPeriodontal diseasespa
dc.subject.keywordsPolymerase Chain Reaction (PCR)spa
dc.subject.keywordsPorphyromonas gingivalisspa
dc.subject.keywordsScaling and root planingspa
dc.titleDetection of specific periodontal microorganisms from bacteraemia samples after periodontal therapy using molecular-based diagnosticsspa
dc.title.translatedDetection of specific periodontal microorganisms from bacteraemia samples after periodontal therapy using molecular-based diagnosticsspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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