Rapid identification of Enterococcus hirae and Enterococcus durans by PCR and detection of a homologue of the E. hirae mur-2 gene in E. durans

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dc.contributor.authorArias, César A.
dc.contributor.authorRobredo Valgañón, Beatriz
dc.contributor.authorSingh, Kavindra V.
dc.contributor.authorTorres, Carmen
dc.contributor.authorpanesso, diana
dc.contributor.authorBeral, Valerie
dc.date.accessioned2021-02-18T19:46:11Z
dc.date.available2021-02-18T19:46:11Z
dc.date.issued2006
dc.description.abstractenglishDuring an evaluation of PCR for identification of isolates of Enterococcus hirae, a homologue with 82% identity to E. hirae mur-2 was identified in Enterococcus durans and was named mur-2ed. PCR using primers for two genes (copY and murG) of E. hirae strains showed amplification with E. hirae strains only. PCR (under high-stringency conditions) with primers for the mur-2ed gene gave the expected amplification product only with E. durans strains. A combination of murG and mur-2ed primers in a multiplex PCR assay differentiated E. hirae from E. durans in all cases. PCR using these primers appears to be a rapid alternative for identification of E. hirae and E. durans isolates.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://dx.doi.org/10.1128%2FJCM.44.4.1567-1570.2006
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1098-660X
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlhttps://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/5379
dc.language.isoeng
dc.publisherAmerican Society for Microbiologyspa
dc.publisher.journalJournal of clinical microbiologyspa
dc.relation.ispartofseriesJournal of clinical microbiology, 1098-660X, Vol. 44, Nro. 4, 2006, p. 1567-1570spa
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1448630/figure/f1/
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2006-02-09
dc.rights.localAcceso abiertospa
dc.subject.decsEnterococcusspa
dc.subject.decsGenoma bacterianospa
dc.subject.decsReacción en cadena de la polimerasaspa
dc.titleRapid identification of Enterococcus hirae and Enterococcus durans by PCR and detection of a homologue of the E. hirae mur-2 gene in E. duransspa
dc.title.translatedRapid identification of Enterococcus hirae and Enterococcus durans by PCR and detection of a homologue of the E. hirae mur-2 gene in E. duransspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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