Evaluation of Allplex™ Entero-DR assay for detection of antimicrobial resistance determinants from bacterial cultures

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dc.contributor.authorMojica, María Fernanda
dc.contributor.authorde la Cadena, Elsa
dc.contributor.authorCorrea, Adriana
dc.contributor.authorAppel, Tobias Manuel
dc.contributor.authorPallares, Christian José
dc.contributor.authorVillegas, María Virginia
dc.contributor.orcidMojica, María Fernanda [0000-0002-1380-9824]
dc.contributor.orcidde la Cadena, Elsa [0000-0003-0361-7893]
dc.contributor.orcidPallares, Christian José [0000-0002-6093-7845]
dc.contributor.orcidVillegas, María Virginia [0000-0003-1898-9067]
dc.date.accessioned2020-03-28T21:21:21Z
dc.date.available2020-03-28T21:21:21Z
dc.date.issued2020
dc.description.abstractenglishObjective To evaluate the sensitivity and specificity of the Allplex™ Entero-DR, a quantitative PCR-based method, for the detection of β-lactamase-encoding genes and vancomycin-resistance determinants in 156 previously characterized Gram-negative bacilli and Enterococcus spp. from bacterial cultures. Result The method had 100% sensitivity and between 92 and 100% of specificity for identifying blaKPC, blaVIM, blaIMP, blaNDM, blaOXA-48-like, blaCTX-M and vanA. In nine isolates, unspecific amplifications were detected. The Ct of these false positives was above 33. The Ct of the correctly identified bla and van genes did not surpass 28 and 30, respectively. None of the clinical isolates included as negative controls yielded any amplification. Therefore, the Allplex™ Entero-DR assay is a highly accurate test for the detection of important antibiotic resistance determinants. With this assay, reliable results can be obtained within 3 h. However, according to our data, samples with Ct values greater than 33 should be considered with caution.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1186/s13104-020-04997-4
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1756-0500
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/2201
dc.publisherSpringer Naturespa
dc.publisher.journalBMC research notesspa
dc.relation.ispartofseriesBMC research notes, 1756-0500, Vol. 13, Nro. 1, 2020, p.1-6spa
dc.relation.urihttps://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-020-04997-4
dc.rightsAttribution 4.0 International*
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf222
dc.rights.creativecommons2020
dc.rights.localAcceso abiertospa
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subject.decsCarbapenémicosspa
dc.subject.decsProductos con acción antimicrobianaspa
dc.subject.decsInformes de casosspa
dc.subject.keywordsMultiplex quantitative PCRspa
dc.subject.keywordsEnterobacteralesspa
dc.subject.keywordsEnteroccocus sppspa
dc.titleEvaluation of Allplex™ Entero-DR assay for detection of antimicrobial resistance determinants from bacterial culturesspa
dc.title.translatedEvaluation of Allplex™ Entero-DR assay for detection of antimicrobial resistance determinants from bacterial cultures
dc.typearticlespa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localartículospa

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