Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolates in Colombia

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dc.contributor.authorChavarro Portillo, Bibiana
dc.contributor.authorMoreno, Jaime Enrique
dc.contributor.authorYomayusa Gonzalez, Nancy
dc.contributor.authorÁlvarez, Carlos Arturo
dc.contributor.authorCastro Cardozo, Betsy Esperanza
dc.contributor.authorEscobar-Pérez, Javier
dc.contributor.authorDíaz, Paula Lucia
dc.contributor.authorIbañez, Milciades
dc.contributor.authorMendez-Alvarez, Sebastián
dc.contributor.authorLEAL, AURA LUCIA
dc.contributor.authorVanegas Gómez, Natasha
dc.contributor.orcidEscobar-Pérez, Javier [0000-0002-0432-6978]
dc.date.accessioned2020-05-12T22:54:16Z
dc.date.available2020-05-12T22:54:16Z
dc.date.issued2013
dc.description.abstractenglishObjective To determine the molecular epidemiology and presence of virulence genes in community-acquired (CA) and hospital-acquired (HA) methicillin-resistant Staphylococcus aureus (MRSA) isolates and their relationship to clinical outcomes. Methods An observational and prospective study of infections caused by MRSA was conducted between June 2006 and December 2007 across seven hospitals in three Colombian cities. MRSA isolates were analyzed for SCCmec. Also, pulsed-field gel electrophoresis and multilocus sequence typing were performed and 25 virulence genes were identified. Results Two hundred and seventy isolates were collected from 262 adult hospital patients with MRSA infections. Overall, 68% of the isolates were classified as HA-MRSA and 32% as CA-MRSA. We identified differences in the patterns of virulence genes: 85% of HA-MRSA isolates possessed the enterotoxin gene cluster (egc), whereas 92% of CA-MRSA isolates possessed the lukF-PV/lukS-PV genes. Multivariate analysis showed an increased risk of mortality for seg (p = 0.001, odds ratio 4.73) and a protective effect for eta (p = 0.018, odds ratio 0.33). Conclusions Our study confirms that three clones of MRSA predominantly circulate in Colombia: a Chilean clone, a pediatric clone that causes HA-MRSA infections, and a USA300-related clone (SCCmec IVc) in CA-MRSA infections, which differ in the content of clinically important virulence genes. This study confirms that PVL is not a determinant of severity or mortality in CA-MRSA infections.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.ijid.2013.02.029
dc.identifier.issn1201-9712
dc.identifier.urihttps://hdl.handle.net/20.500.12495/2660
dc.language.isoeng
dc.publisherElsevier BVspa
dc.publisher.journalInternational Journal of Infectious Diseasesspa
dc.relation.ispartofseriesInternational Journal of Infectious Diseases, 1201-9712, Vol.17, Nro.9, 2013, p.e744-e749spa
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S1201971213001227
dc.rights.creativecommons2013
dc.rights.localAcceso cerradospa
dc.subject.decsTipificación de secuencias multilocusspa
dc.subject.decsElectroforesis en gel de campo pulsadospa
dc.subject.decsFactores de virulenciaspa
dc.subject.keywordsStaphylococcus aureusspa
dc.subject.keywordsCA-MRSAspa
dc.subject.keywordsAntibiotic resistancespa
dc.subject.keywordsEnterotoxinsspa
dc.titleMolecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolates in Colombiaspa
dc.typearticlespa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localartículospa

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