Multilocus genotyping of Giardia intestinalis in pet dogs of Medellín Colombia

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dc.contributor.authorHernández, Paula C.
dc.contributor.authorMorales de la Pava, Liliana
dc.contributor.authorChaparro-Olaya, Jacqueline
dc.contributor.authorLópez-Osorio, Sara
dc.contributor.authorLópez-Arias, Ánderson
dc.contributor.authorChaparro-Gutiérrez, Jenny Jovana
dc.date.accessioned2021-03-02T15:04:01Z
dc.date.available2021-03-02T15:04:01Z
dc.date.issued2021
dc.description.abstractenglishAccording to a few parasitological and epidemiological studies, Giardia is the most prevalent parasitic infection among pet dogs in the city of Medellín, the second-largest city in Colombia. This study determined the assemblages of Giardia in the fecal samples of dogs obtained from 18 veterinary centers of Medellín. One hundred fecal samples of dogs diagnosed with Giardia using microscopy were analyzed via nested polymerase chain reaction (PCR) using three genes (gdh, bg, and tpi). The PCR products were purified and sequenced, and phylogenetic analyses were conducted using the maximum likelihood algorithm of the three loci. From the 100 samples analyzed, 47 were Giardia-positive via PCR. Genotypes C and D were detected in six samples, neither of which were associated with human infection. However, the zoonotic potential of Giardia cannot be ruled out because of the small number of samples that could be sequenced for assemblage assignation.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.vprsr.2020.100520
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn2405-9390
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/5502
dc.language.isoeng
dc.publisherElsevierspa
dc.publisher.journalVeterinary Parasitology: Regional Studies and Reportsspa
dc.relation.ispartofseriesVeterinary Parasitology: Regional Studies and Reports, 2405-9390, Vol. 23, p. 1-6spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S2405939020303014
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2021-01
dc.rights.localAcceso abiertospa
dc.subject.keywordsGiardia intestinalisspa
dc.subject.keywordsGenetic assemblagesspa
dc.subject.keywordsPet dogsspa
dc.subject.keywordsMultilocus genotypingspa
dc.subject.keywordsColombiaspa
dc.titleMultilocus genotyping of Giardia intestinalis in pet dogs of Medellín Colombiaspa
dc.title.translatedMultilocus genotyping of Giardia intestinalis in pet dogs of Medellín Colombiaspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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