The effects of Sarconesiopsis magellanica larvae (Diptera: Calliphoridae) excretions and secretions on fibroblasts

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dc.contributor.authorPinilla, Yudi T.
dc.contributor.authorPatarroyo, Manuel-Alfonso
dc.contributor.authorVelandia-Romero, Myriam Lucía
dc.contributor.authorSegura, Nidya A.
dc.contributor.authorBello, Felio J.
dc.contributor.orcidVelandia-Romero, Myriam Lucía [0000-0002-3340-7304]
dc.date.accessioned2020-05-13T19:40:31Z
dc.date.available2020-05-13T19:40:31Z
dc.date.issued2015
dc.description.abstractenglishSarconesiopsis magellanica is a necrophagous blowfly which is relevant in both forensic and medical sciences. Previous studies regarding this species have led to understanding life-cycle, population and reproduction parameters, as well as identifying and characterising proteolytic enzymes derived from larval excretions and secretions (ES). As other studies have shown that ES proteolytic activity plays a significant role in wound healing and fibroblasts play a relevant role in granulation tissue formation during such healing, the present study was aimed at analysing the biological effect of S. magellanica larval ES on fibroblasts. ES were obtained from third-instar larvae and added to fibroblast cells at three concentrations (10, 5 and 1 μg/mL) to evaluate their behaviour. MTT assays were used for analysing cell proliferation and viability, whilst cell adhesion was measured by optical density with 10% SDS. Fibroblast migration and morphology was recorded by microscopic observation. ES did not affect fibroblast viability and induced an increase in cell proliferation; cell adhesion became reduced, whilst cell migration through extracellular matrix increased. ES also induced a decreased cell surface and morphological alterations. Changes in all the above-mentioned parameters were reduced when ES were incubated at 60 °C, probably due to protease denaturation. These results suggested that the proteases contained in S. magellanica larval ES contributed towards granulation tissue formation, increased cell migration and promoted cell proliferation. All these data support carrying out further experiments aimed at validating S. magellanica usefulness in larval therapy.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.actatropica.2014.11.003
dc.identifier.issn0001-706X
dc.identifier.urihttps://hdl.handle.net/20.500.12495/2703
dc.language.isoeng
dc.publisherElsevier BVspa
dc.publisher.journalActa Tropicaspa
dc.relation.ispartofseriesActa Tropica, 0001-706X, Vol. 142, 2015, p. 26-33spa
dc.relation.urihttps://www.sciencedirect.com/science/article/abs/pii/S0001706X14003465
dc.rights.creativecommons2015
dc.rights.localAcceso cerradospa
dc.subject.decsFibroblastosspa
dc.subject.decsMovimiento celularspa
dc.subject.decsProliferación celularspa
dc.subject.keywordsSarconesiopsis magellanicaspa
dc.subject.keywordsProteolytic enzymespa
dc.subject.keywordsLarval therapyspa
dc.subject.keywordsLarval excretionspa
dc.titleThe effects of Sarconesiopsis magellanica larvae (Diptera: Calliphoridae) excretions and secretions on fibroblastsspa
dc.typearticlespa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localartículospa

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