Comparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cells

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dc.contributor.authorAvila-Portillo, L. M.
dc.contributor.authorAristizabal, F.
dc.contributor.authorPerdomo Lara, Sandra Janneth
dc.contributor.authorRiveros, A.
dc.contributor.authorOspino, B.
dc.contributor.authorAvila, J. P.
dc.contributor.authorButti, M.
dc.contributor.authorAbba, M. C.
dc.contributor.orcidPerdomo Lara, Sandra Janneth [0000-0002-4429-3760]
dc.date.accessioned2021-02-09T17:05:15Z
dc.date.available2021-02-09T17:05:15Z
dc.date.issued2020
dc.description.abstractenglishBiosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways. Umbilical cord blood cells-derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs. In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1177/1177932220913307
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1177-9322
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlhttps://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/5278
dc.language.isoeng
dc.publisherSage Journalsspa
dc.publisher.journalBioinformatics & Biology Insightsspa
dc.relation.ispartofseriesBioinformatics & Biology Insights, 1177-9322, Vol. 14, 2020, p. 1-7spa
dc.relation.urihttps://journals.sagepub.com/doi/10.1177/1177932220913307
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightsAcceso abierto
dc.rights.creativecommons2020-03-20
dc.rights.localAcceso abiertospa
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/*
dc.subject.keywordsG-CSFspa
dc.subject.keywordsBiosimilarspa
dc.subject.keywordsInnovatorspa
dc.subject.keywordsTranscriptomicsspa
dc.subject.keywordsUmbilical cord bloodspa
dc.titleComparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cellsspa
dc.title.translatedComparative analysis of the biosimilar and innovative G-CSF modulated pathways on umbilical cord blood–derived mononuclear cellsspa
dc.type.coarhttps://purl.org/coar/resource_type/c_6501
dc.type.driverinfo:eu-repo/semantics/article
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localArtículo de revista

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