Comparación del microbioma bacteriano intestinal a partir de aspirado de colon, íleon y de materia fecal de pacientes con espondiloartritis

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dc.contributor.advisorMaquez Ortiz , Ricaurte Alejandro
dc.contributor.advisorRomero Sánchez, María Consuelo
dc.contributor.authorLeón Falla, Moises David
dc.date.accessioned2024-08-08T12:58:06Z
dc.date.available2024-08-08T12:58:06Z
dc.date.issued2024-07
dc.description.abstractEl estudio del microbioma del tracto gastrointestinal (TGI) de pacientes con Espondiloartritis (EspA) se ha enfocado en el análisis de muestras de materia fecal, las cuales reflejan principalmente el microbioma del lumen intestinal. El objetivo de este estudio fue describir y comparar el microbioma luminal y de la mucosa intestinal de pacientes con EspA, utilizando aspirados de lavados obtenidos de colonoscopia (ALC), una reciente alternativa para el estudio de estas regiones en el TGI. Se analizaron 59 ALC (de íleon distal y colon sigmoide) y 41 muestras de materia fecal de 32 pacientes con EspA y 7 individuos sanos utilizando un perfilamiento metataxonómico dirigido al gen 16S RNAr. El perfilamiento metatoxinomico confirmó que las muestras de ALC del TGI inferior (íleon y colon) presentaron un microbioma indiferenciado, distintivo y separado del que se halló en las muestras de materia fecal o en el inicio del TGI representado por cavidad oral (CO). Las muestras del TGI bajo y materia fecal de pacientes con EspA exhibieron un comportamiento similar al del microbioma del grupo con Enfermedad Inflamatoria Intestinal (EII), con una reducción en la riqueza y diversidad microbiana comparadas con las de los controles sanos. Curiosamente, se evidenció un incremento de taxones proinflamatorios en los pacientes con EspA como la familia Enterobacteriaceae (principalmente en el íleon), Succinivibrio spp. y Prevotella stercorea. Por otro lado, los pacientes con EspA presentaron una disminución significativa de productores de Ácidos Grasos de Cadena Corta (AGCC) Coprococcus catus y Eubacterium biforme. Los datos obtenidos respaldaron el valor de las muestras de ALC para el estudio del TGI y contribuyeron con información de “taxones disruptores” involucrados en los desórdenes asociados al TGI observados en pacientes con EspA.
dc.description.abstractenglishThe study of the Gastrointestinal tract (GIT) microbiome of Spondyloarthritis (SpA) patients has focused on the analysis of feces samples, that picture mostly the luminal microbiota. The aim of this study was to describe and compare the luminal and intestinal mucosa microbiome in SpA, using colonoscopy aspiration lavages (CAL), a recent alternative for regional studies of the GIT. We analyzed 59 CAL (from sigmoid colon and distal ileum), and 41 feces samples, from 32 SpA patients and 7 healthy individuals, using 16S rRNA gene-targeted metataxonomic profiling. Metataxonomic profiling confirmed CAL samples from the lower GIT (colon or ileum) presented a distinctive and undifferentiated bacteriome and separate from that found in fece’s samples or in the beginning of the GIT (oral cavity (OC)). Lower GIT samples and feces of SpA patients exhibited similar behavior to the microbiome of IBD group with reduced microbial richness and diversity, comparing to the healthy controls. Interestingly, it was found increase in proinflammatory taxa in SpA patients, such as Enterobacteriaceae family (mostly in the ileum), Succinivibrio spp. and Prevotella stercorea. Conversely, SpA patients presented significant decrease in the Short-Chain Fatty Acid (SCFA) producers Coprococcus catus and Eubacterium biforme. Our data support the value of CAL samples for the regional study of GI-tract and contribute with information of potential “disruptor taxa” involved in the GI-tract associated disorders observed in SpA patients.
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias Básicas Biomédicasspa
dc.format.mimetypeapplication/pdf
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/12844
dc.language.isoes
dc.publisher.facultyFacultad de Medicinaspa
dc.publisher.grantorUniversidad El Bosquespa
dc.publisher.programMaestría en Ciencias Básicas Biomédicasspa
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dc.rightsAttribution 4.0 Internationalen
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf2
dc.rights.localAcceso abiertospa
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAspirados
dc.subjectColonoscopia
dc.subjectMateria fecal,
dc.subjectMicrobioma
dc.subjectDisbiosis
dc.subjectRNAr 16S
dc.subjectTracto gastrointestinal
dc.subjectEspondiloartritis
dc.subject.keywordsColonoscopy
dc.subject.keywordsAspiration lavages
dc.subject.keywordsfeces
dc.subject.keywordsMicrobiome
dc.subject.keywordsDysbiosis
dc.subject.keywords16S rRNA
dc.subject.keywordsGastrointestinal tract
dc.subject.keywordsSpondyloarthritis
dc.subject.nlmW 50
dc.titleComparación del microbioma bacteriano intestinal a partir de aspirado de colon, íleon y de materia fecal de pacientes con espondiloartritis
dc.title.translatedComparison of the intestinal bacterial microbiome from colon and ileum aspirate and feces from patients with spondyloarthritis
dc.type.coarhttps://purl.org/coar/resource_type/c_bdcc
dc.type.coarversionhttps://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.driverinfo:eu-repo/semantics/masterThesis
dc.type.hasversioninfo:eu-repo/semantics/acceptedVersion
dc.type.localTesis/Trabajo de grado - Monografía - Maestríaspa

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