• Browse
    • Login
    View Item 
    •   DSpace Home
    • Investigación
    • Artículos
    • View Item
    •   DSpace Home
    • Investigación
    • Artículos
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Whole-genome analyses of enterococcus faecium isolates with diverse daptomycin MICs

    Thumbnail
    View/Open
    Diaz_Lorena_2014.pdf (1.116Mb)
    Share this
    TY - GEN T1 - Whole-genome analyses of enterococcus faecium isolates with diverse daptomycin MICs UR - http://hdl.handle.net/20.500.12495/3553 PB - American Society for Microbiology AB - ER - @misc{20.500.12495_3553, author = {}, title = {Whole-genome analyses of enterococcus faecium isolates with diverse daptomycin MICs}, year = {}, abstract = {}, url = {http://hdl.handle.net/20.500.12495/3553} }RT Generic T1 Whole-genome analyses of enterococcus faecium isolates with diverse daptomycin MICs LK http://hdl.handle.net/20.500.12495/3553 PB American Society for Microbiology AB OL Spanish (121)
    Gestores bibliográficos
    Refworks
    Zotero
    BibTeX
    CiteULike
    Author
    Diaz, Lorena 
    Tran, Truc T
    Munita, Jose M 
    Miller, William R 
    Rincon, Sandra 
    Carvajal, Lina P
    Wollam, Aye
    Reyes, Jinnethe 
    Panesso, Diana 
    Rojas, Natalia L
    Shamoo, Yousif 
    Murray, Barbara E
    Weinstock, George M
    Arias, Cesar A 
    Date
    2014-08
    Published in
    Antimicrobial Agents and Chemotherapy, 1098-6596, Vol. 58, No. 8, 2014 p. 4527-4534
    Published for
    American Society for Microbiology
    URI
    http://hdl.handle.net/20.500.12495/3553
    Source's URL
    https://aac.asm.org/content/58/8/4527.long
    DOI
    https://doi.org/10.1128/aac.02686-14

    Citación

         
    Metadata
    Show full item record
    Documents PDF
    Abstract
    Daptomycin (DAP) is a lipopeptide antibiotic frequently used as a "last-resort" antibiotic against vancomycin-resistant Enterococcus faecium (VRE). However, an important limitation for DAP therapy against VRE is the emergence of resistance during therapy. Mutations in regulatory systems involved in cell envelope homeostasis are postulated to be important mediators of DAP resistance in E. faecium. Thus, in order to gain insights into the genetic bases of DAP resistance in E. faecium, we investigated the presence of changes in 43 predicted proteins previously associated with DAP resistance in enterococci and staphylococci using the genomes of 19 E. faecium with different DAP MICs (range, 3 to 48 μg/ml). Bodipy-DAP (BDP-DAP) binding to the cell membrane assays and time-kill curves (DAP alone and with ampicillin) were performed. Genetic changes involving two major pathways were identified: (i) LiaFSR, a regulatory system associated with the cell envelope stress response, and (ii) YycFGHIJ, a system involved in the regulation of cell wall homeostasis. Thr120 → Ala and Trp73 → Cys substitutions in LiaS and LiaR, respectively, were the most common changes identified. DAP bactericidal activity was abolished in the presence of liaFSR or yycFGHIJ mutations regardless of the DAP MIC and was restored in the presence of ampicillin, but only in representatives of the LiaFSR pathway. Reduced binding of BDP-DAP to the cell surface was the predominant finding correlating with resistance in isolates with DAP MICs above the susceptibility breakpoint. Our findings suggest that genotypic information may be crucial to predict response to DAP plus β-lactam combinations and continue to question the DAP breakpoint of 4 μg/ml.
    Topics
    Enterococcus faecium
    Daptomicina
    Vancomicina
    Collections
    • Artículos [1458]

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister
    GTM statisticsGTM statistics
    Contacto Repositorio Institucional

    Teléfono: (571) 648 9000 Ext: 1546 - Correo: adminrepositorio@unbosque.edu.co

    Universidad El Bosque

    Instalaciones Bogotá

    Av. Cra. 9 No. 131 A - 02
    Línea gratuita:
    018000 113033
    PBX: (571) 648 9000

    Sede

    Instalaciones Chía

    Autopista Norte
    Km. 20 costado occidental
    Vía Chía - Bogotá
    Teléfono: 676 3110
    Línea gratuita: 018000 113033
    PBX: (571) 648 9000

    Sede Chía

    Admisiones

    PBX.: 648 9000
    Línea gratuita:
    018000 113033
    Edificio Fundadores
    Av. Cra. 9 No. 131 A - 02
    Skype: /uelbosque
    Correo electrónico:
    atencionalusuario@unbosque.edu.co

    Participante

    ACE Internationalization Lab

    Información Legal

    • Valores Pecuniarios
    • Estatuto General
    • Reglamento Estudiantil
    • Estatuto Docente
    • Política de Bienestar Universitario
    • Política de Tratamiento de Datos Personales
    • Términos y Condiciones de Uso del Sitio
    • Aviso de Privacidad
    • Información Tributaria

    Vigilada Mineducación. Personería Jurídica otorgada mediante resolución 11153 del 4 de agosto de 1978.