Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells
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Fecha
2019
Título de la revista
Publicado en
Journal of supercritical fluids, 0896-8446, Vol. 15, 2019
Publicado por
Elsevier
ISSN de la revista
Título del volumen
Resumen
Descripción
Abstract
Mango seed kernel(MSK) has demonstrated to be a valuable source of bioactive phenolic compounds with
antiproliferative activity against colon cancer cell lines. In this work, a phenolic MSK extract obtained
by sequential pressurized liquid extraction (PLE) was submitted to supercritical antisolvent fractionation (SAF) to attain enriched fractions with improved inhibitory effect on HT-29 colon cancer cells.
SAF was optimized using a Box-Behnken experimental design and considering as main factors: pressure, percentage of water in the feed extract and feed/sc−CO2 flow rate ratio. The highest inhibitory cell
proliferation effect (70.51 ± 1.14%) was obtained in the extract using 50% of water v/v in the feed and
0.0625 feed/sc−CO2 ratio at 15 MPa. A targeted phytochemical profiling by LC-q-TOF-MS/MS followed
by a multivariate statistical analysis of the observed bioactivity and the chemical composition evidenced
the presence of characteristic compounds which might explain the enhanced antiproliferative activity of
the optimal SAF extract.
Palabras clave
Keywords
Mangifera indica L, Fruit by-products, Supercritical antisolvent fractionation
Temáticas
Espectrometría de masas
Análisis químico
Dióxido de carbono
Análisis químico
Dióxido de carbono