Frecuencias alélicas y genotípicas de polimorfismos de genes asociados a la vía de Wnt en individuos sistémicamente sanos y su asociación con variables clínicas periodontales
Ospina Chavarro, María Victoria
Lafaurie Villamil, Gloria Ínes
Degree nameEspecialista en periodoncia y medicina oral
ProgramEspecialización en periodoncia y medicina oral
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There is a limited number of studies which evaluate SNPs associated with the Wnt pathway of modulation and osseous resorption on systemically healthy subjects and its relation with the presence of periodontitis. These have described the relation of periodontitis and its severity with other pathways which regulate osseous metabolism such as RANK- RANKLOPG; this leads to the need of evaluating the possible relation with other pathways with similar control and metabolism functions of osseous mass. To establish the genotype and allelic frequencies of gene polymorphs associated with the wnt pathway on healthy subjects with and without periodontal disease. Sub-gingival plaque samples, periodontal indices, PD diagnosis with CDC and AAP, periodontal probing, insertion levels, serology and clinical inflammation indices were taken from systemically healthy individuals with and without PD. The SNPs of DKK, SOST, LRP5 and KREMEN were determined with High Resolution Melting and confirmed with sequencing. 57.3% of the population presented PD with 9.6% being severe. 67.2% presented SNP for DKKrs1896367 in the group without PD (p=0.016). 85.6% of individuals with PD presented SNPs for DKKrs1896368 (p=0.042), place frequencies for each polymorphism. The allelic frequencies of DKKrs1896368 such as 0.435 (42.35%) for T ancestral alleles and 181 alleles which represent the 0.5764 (57.64%). DKKrs1896367 and DKKrs1896368 are thus associated with PD. All individuals with SNP DKKrs1896368 had severe PD and DKKrs1896367 and kremenrs132274 were present in those without PD. These are important initial findings regarding the presence of PD in the Wnt pathway.
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