Identificación de genes de resistencia antibiótica tetQ, tetM, qnrS, qnrB en bacilos entéricos aislados de cavidad oral
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2019
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: Enteric bacteria are considered transient micro-biota in the oral cavity and its distribution vary depending on diverse factors. They have been associated with an efficiency reduction of oral treatments, which relates to higher resistance rates and an important reservoir of antibiotic resistant genes. Even though there have been several approaches evaluating different genes, there is still very scarce literature on the oral topic, mainly among Colombian population. Objective: to establish the frequency of the antibiotic resistance genes tetQ, tetM, qnrS, qnrB in enteric bacilli isolated
from saliva samples. Materials and methods: A sample of 100 isolations from saliva samples were selected with gender and bacterial species identified using biochemical galleries, PCR for detection of tetM and tetQ associated with a resistance to doxycycline and qnrB and qnrS associated with resistance to ciprofloxacin. Results: The most frequent gene was tetM
with a 52% mainly in the Enterobacter (56%) and Klebsiella (85%) genders, followed by qnrB detected 17% in Klebsiella (53%) and Cronobacter (23%). The detection of tetQ and qnrS genes was low (3% and 1% respectively). Conclusions: Enteric bacilli do not make part of normal oral microbiota, they may be considered important reservoirs of resistant genes to antibiotics of primary use in dentistry, as well as alternative treatments.
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Las bacterias entéricas son consideradas microbiota transeúnte en cavidad oral y su distribución en la población varía, dependiendo de diversos factores. Se han asociado con la reducción de la efectividad en los tratamientos orales, lo cual se ha relacionado con las altas tasas de resistencia que presentan y su papel como reservorio importante de muchos genes de resistencia a antibióticos. Aunque se han realizado varios acercamientos evaluando diversos genes, a nivel oral aún se encuentra muy poca literatura que aporte información acerca del tema, principalmente a nivel de la población colombiana. Establecer la frecuencia de los genes de resistencia antibiótica tetQ, tetM, qnrS, qnrB en bacilos entéricos aislados a partir de muestras de saliva. Se seleccionan 100 aislamientos de bacilos entéricos provenientes de muestras de saliva, se realizó identificación de género y especie bacteriana utilizando galerías bioquímicas, y PCR para detección de los genes tetM y tetQ, asociados con resistencia a doxiciclina, y qnrB y qnrS asociados con resistencia a ciprofloxacina. Se observó que el gen encontrado en mayor frecuencia fue tetM en un 52%, principalmente en los géneros Enterobacter (56%) y Klebsiella (85%), seguido de qnrB, el cual se detectó en un 17%, encontrándose en Klebsiella en un 35%, y en Cronobacter en un 23%. Los genes tetQ y qnrS fueron detectados en baja frecuencia (3% y 1% respectivamente). Aunque los bacilos entéricos no hacen parte de la microbiota normal de la cavidad oral pueden ser considerados reservorios importantes de genes de resistencia a antibióticos que son empleados en odontología no solo en los de primera elección, sino también en los que se utilizan como tratamiento alternativo.
Abstract
Enteric bacteria are considered transient micro-biota in the oral cavity and its distribution vary depending on diverse factors. They have been associated with an efficiency reduction of oral treatments, which relates to higher resistance rates and an important reservoir of antibiotic resistant genes. Even though there have been several approaches evaluating different genes, there is still very scarce literature on the oral topic, mainly among Colombian population. To establish the frequency of the antibiotic resistance genes tetQ, tetM, qnrS, qnrB in enteric bacilli isolated from saliva samples. A sample of 100 isolations from saliva samples were selected with gender and bacterial species identified using biochemical galleries, PCR for detection of tetM and tetQ associated with a resistance to doxycycline and qnrB and qnrS associated with resistance to ciprofloxacin. The most frequent gene was tetM with a 52% mainly in the Enterobacter (56%) and Klebsiella (85%) genders, followed by qnrB detected 17% in Klebsiella (53%) and Cronobacter (23%). The detection of tetQ and qnrS genes was low (3% and 1% respectively). Enteric bacilli do not make part of normal oral microbiota, they may be considered important reservoirs of resistant genes to antibiotics of primary use in dentistry, as well as alternative treatments.
Palabras clave
Resistencia antibiótica, Bacilos entéricos, Cavidad oral
Keywords
Antibiotic resistance, Enteric bacilli, Oral cavity
Temáticas
Resistencia a medicamentos
Enterobacteriaceae
Boca
Enterobacteriaceae
Boca