Examinando por Autor "Duque Varela, Juan Pablo"

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  • Miniatura
    Ítem
    Carga de enfermedad por cáncer de ovario en Colombia durante el periodo 2015-2020
    (2023-08) Duque Varela, Juan Pablo; Meneses Gil, María Ximena; Porras Ramírez, Alexandra; Duque Varela, Juan Pablo [0000-0003-0980-3275]; Meneses Gil, Maria Ximena [0000-0002-5339-8927]
    Determinar la carga de enfermedad por cáncer de ovario en Colombia entre el periodo de 2015-2020 a través de los años de vida ajustados por discapacidad (DALYs). Materiales y métodos: estudio de carga de enfermedad para determinar los DALYs producidos por cáncer de ovario en Colombia con pesos de discapacidad de 0.25 y 1.0. La unidad de análisis fueron los registros de mortalidad del Departamento Administrativo Nacional de Estadísticas DANE de acuerdo al código CIE10 (C56X). Resultados. La morbilidad y mortalidad entre 2015-2020 en Colombia por cáncer de ovario presentó una tendencia al incremento. La tendencia más alta se encuentra en el grupo de 60-69 años. Discusión. La carga de enfermedad por cáncer de ovario en Colombia ha aumentado en los últimos años, presentando un pico significativo y de discapacidad en el año 2019.
  • Miniatura
    Ítem
    Estandarización de una metodología para la cuantificación de títulos virales por citometría de flujo: virus dengue y zika
    (Universidad El Bosque) Almanza Holguín, Laura Natalia; Duque Varela, Juan Pablo; Delgado Tiria, Felix Giovanni; Delgado Tiria, Felix Giovanni [0000-0001-6685-7507]
    The basic research in virology has a Standard Gold methodology for the calculation of viral titer which is the plating technique. However, this technique requires a wide availability of time and experiences by the experimenter; Therefore, proposing an alternative and equally reliable technique is a challenge that currently exists. For this reason, this project aims to statistically compare the results obtained between the traditional method and flow cytometry to see if it is feasible to use the latter as a routine technique in the quantification of viral loads. For this, three different cell lines (VERO, BHK-21 and U937-DC-SIGN) and the dengue (4 serotypes) and Zika viruses were used in a new protocol for flow cytometry which is based on the use of 2-fold serial dilutions of viral inoculum in order to obtain percentages of infected cells and then calculate the viral titers. The results obtained by flow cytometry were compared with those previously obtained by plaque assay, applying a correlation analysis. As a result, it was evident that the viral titers calculated using the BHK-21 cells correlated significantly with those reported by the plaque assay. Additionally, it was observed that the infectivity curves were different between the different cell lines used, showing different degrees of susceptibility to infection at the time of the test. In conclusion, our results show that the flow cytometry technique can be used as an alternative method for the quantification of viral particles, obtaining results that correlate with those obtained by plating, as long as BHK-21 cells are used.