Frecuencia de ANCA positivos en una población con síntomas clínicos sugestivos de enfermedad autoinmune y la interferencia de ANA en su interpretación

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dc.contributor.authorRomero-Sánchez, Consuelo
dc.contributor.authorBenavides-Solarte, Mario
dc.contributor.authorGalindo-Ibáñez, Isabel
dc.contributor.authorOspina-Caicedo, Ana Isabel
dc.contributor.authorParra-Izquierdo, Viviana
dc.contributor.authorChila-Moreno, Lorena
dc.contributor.authorVilla, Amanda
dc.contributor.authorCasas-Gómez, María Consuelo
dc.contributor.authorAngarita, Ignacio
dc.contributor.authorBautista-Molano, Wilson Armando
dc.contributor.authorRomero-Álvarez, Verónica
dc.contributor.authorBello-Gualtero, Juan Manuel
dc.contributor.orcidRomero-Sánchez, Consuelo [0000-0002-6973-7639]
dc.contributor.orcidBautista-Molano, Wilson Armando [0000-0003-0684-9542]
dc.contributor.orcidBautista-Molano, Wilson Armando [0000-0003-0684-9542]
dc.contributor.orcidBautista-Molano, Wilson Armando [0000-0003-0684-9542]
dc.date.accessioned2020-05-18T11:38:40Z
dc.date.available2020-05-18T11:38:40Z
dc.date.issued2019
dc.description.abstractenglishBACKGROUND: Antibodies against neutrophil cytoplasm (ANCA) are associated with vasculitis. There are different methods to determine their presence. The interference of antinuclear antibodies (ANA) in the differentiation between P-ANCA and C-ANCA patterns has been described. OBJECTIVE: To determine the frequency of ANCA in a population with manifestations of autoimmune disease, and evaluate the interference of ANA in its interpretation. MATERIALS AND METHODS: Retrospective, descriptive nonexperimental cross-sectional study, including 3,330 data. The presumptive diagnosis was autoimmune disease and a test for ANCA was requested. The ANCA and ANA determinations were made by indirect immunofluorescence, L-ANCA® and CytoBead® ANCA. Anti-proteinase 3 and anti-myeloperoxidase were detected by ELISA and CytoBead® ANCA. RESULTS: ANCAs were positive in 10.21% and 12.64% of those positive for ANCA were positive for ANA. The inter-rater agreement statistic (Kappa) for anti-PR3 between CytoBead ANCA and ELISA was 100% (K=1.00; P<.05) and the agreement between anti- myeloperoxidase by ELISA and CytoBead® ANCA was high (K=0.94; P<.05). 30% of those with ANCAs had a diagnosis of a type of vasculitis; 20% of them had an autoimmune disease. CONCLUSIONS: The results suggest an overestimated request for ANCAs as a diagnostic aid in primary care which was not addressed. For an adequate evaluation of ANCAs, the indirect immunofluorescence technique should be implemented for the control and confirmation with the determination of specific antigens for anti- proteinase 3 and anti- myeloperoxidase in any of the confirmatory assays. The high concordance shown by ANCA CytoBeads makes us consider the use of this alternative for the determination of ANCAs and the confirmation. Given the interference of ANAs, the ANA test by IFI in the presence of positive P-ANCA results is recommended in order to minimize "false positives".eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.reuma.2018.09.007
dc.identifier.issn1699-258X
dc.identifier.urihttps://hdl.handle.net/20.500.12495/2946
dc.language.isospa
dc.language.isoeng
dc.publisherSociedad Española de Reumatologíaspa
dc.publisher.journalReumatología Clínicaspa
dc.relation.ispartofseriesReumatología Clínica, 1699-258X, 2019spa
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S1699258X18302213?via%3Dihub
dc.rights.creativecommons2019
dc.rights.localAcceso cerradospa
dc.subjectAnticuerpos anticitoplasma de neutrófilosspa
dc.subjectAnticuerpos antinuclearesspa
dc.subject.keywordsAntineutrophil cytoplasmic antibodiesspa
dc.subject.keywordsAntinuclear antibodiesspa
dc.subject.keywordsVasculitisspa
dc.titleFrecuencia de ANCA positivos en una población con síntomas clínicos sugestivos de enfermedad autoinmune y la interferencia de ANA en su interpretaciónspa
dc.title.translatedFrequency of positive ANCA test in a population with clinical symptoms suggestive of autoimmune disease and the interference of ANA in its interpretationspa
dc.typearticlespa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localartículospa

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