Supercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cells

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dc.contributor.authorBallesteros Vivas, Diego
dc.contributor.authorAlvarez, Gerardo
dc.contributor.authorGarcía Ocampo, Andrés Felipe
dc.contributor.authorMorantes Medina, Sandra Johanna
dc.contributor.authorSánchez-Camargo, Andrea del Pilar
dc.contributor.authorCifuentes, Alejandro
dc.contributor.authorParada-Alfonso, Fabián
dc.contributor.authorIbanez, Elena
dc.date.accessioned2020-05-06T23:45:18Z
dc.date.available2020-05-06T23:45:18Z
dc.date.issued2019
dc.description.abstractenglishMango seed kernel(MSK) has demonstrated to be a valuable source of bioactive phenolic compounds with antiproliferative activity against colon cancer cell lines. In this work, a phenolic MSK extract obtained by sequential pressurized liquid extraction (PLE) was submitted to supercritical antisolvent fractionation (SAF) to attain enriched fractions with improved inhibitory effect on HT-29 colon cancer cells. SAF was optimized using a Box-Behnken experimental design and considering as main factors: pressure, percentage of water in the feed extract and feed/sc−CO2 flow rate ratio. The highest inhibitory cell proliferation effect (70.51 ± 1.14%) was obtained in the extract using 50% of water v/v in the feed and 0.0625 feed/sc−CO2 ratio at 15 MPa. A targeted phytochemical profiling by LC-q-TOF-MS/MS followed by a multivariate statistical analysis of the observed bioactivity and the chemical composition evidenced the presence of characteristic compounds which might explain the enhanced antiproliferative activity of the optimal SAF extract.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1016/j.supflu.2019.104563
dc.identifier.issn0896-8446
dc.identifier.urihttps://hdl.handle.net/20.500.12495/2483
dc.language.isoeng
dc.publisherElsevierspa
dc.publisher.journalJournal of supercritical fluidsspa
dc.relation.ispartofseriesJournal of supercritical fluids, 0896-8446, Vol. 15, 2019spa
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0896844619302852?via%3Dihub
dc.rights.creativecommons2019
dc.rights.localAcceso cerradospa
dc.subject.decsEspectrometría de masasspa
dc.subject.decsAnálisis químicospa
dc.subject.decsDióxido de carbonospa
dc.subject.keywordsMangifera indica Lspa
dc.subject.keywordsFruit by-productsspa
dc.subject.keywordsSupercritical antisolvent fractionationspa
dc.titleSupercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cellsspa
dc.title.translatedSupercritical antisolvent fractionation as a tool for enhancing antiproliferative activity of mango seed kernel extracts against colon cancer cellsspa
dc.typearticlespa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localartículospa

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DiegoBallesteros-Vivasab1GerardoAlvarez-Rivera.Andrés FelipeGarcíaOcampo,Sandra JohannaMorantes,Andrea del PilarSánchez Camargo,AlejandroCifuentesbFabiánParada-AlfonsoaElenaIbánez_2019.pdf
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