Detection of giardia duodenalis antigen in human fecal eluates by enzyme-linked immunosorbent assay using polyclonal antibodies

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dc.contributor.authorDuque Beltrán, Sofía
dc.contributor.authorNicholls Orejuela, Rubén Santiago
dc.contributor.authorArévalo Jamaica, Adriana
dc.contributor.authorGuerrero-Lozano, Rafael
dc.contributor.authorMontenegro, Sonia
dc.contributor.authorJames, Mark A
dc.date.accessioned2019-08-27T16:40:13Z
dc.date.available2019-08-27T16:40:13Z
dc.date.issued2002
dc.description.abstractenglishThe present study developed and standardized an enzime-linked immunosorbent assay (ELISA) to detect Giardia antigen in feces using rabbit polyclonal antibodies. Giardia cysts were purified from human fecal samples by sucrose and percoll gradients. Gerbils (Meriones unguiculatus) were infected to obtain trophozoites. Rabbits were inoculated with either cyst or trophozoite antigens of 14 Colombian Giardia isolates to develop antibodies against the respective stages. The IgG anti-Giardia were purified by sequential caprylic acid and ammonium sulfate precipitation. A portion of these polyclonal antibodies was linked to alkaline phosphatase (conjugate). One hundred and ninety six samples of human feces, from different patients, were tested by parasitologic diagnosis: 69 were positive for Giardia cysts, 56 had no Giardia parasites, and 71 revealed parasites other than Giardia. The optimal concentration of polyclonal antibodies for antigen capture was 40 µg/ml and the optimal conjugate dilution was 1:100. The absorbance cut-off value was 0.24. The parameters of the ELISA test for Giardia antigen detection were: sensitivity, 100% (95% CI: 93.4-100%); specificity, 95% (95% CI: 88.6-97.6%); positive predictive value, 91% (95% CI: 81.4-95.9%); and negative predictive value, 100% (95% CI: 96.1-100%). This ELISA will improve the diagnosis of Giardia infections in Colombia and will be useful in following patients after treatment.eng
dc.format.mimetypeapplication/pdf
dc.identifier.doihttps://doi.org/10.1590/S0074-02762002000800018
dc.identifier.instnameinstname:Universidad El Bosquespa
dc.identifier.issn1678-8060
dc.identifier.reponamereponame:Repositorio Institucional Universidad El Bosquespa
dc.identifier.repourlrepourl:https://repositorio.unbosque.edu.co
dc.identifier.urihttps://hdl.handle.net/20.500.12495/1661
dc.language.isoeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúdespa
dc.publisher.journalMemorias do Instituto Oswaldo Cruzspa
dc.relation.ispartofseriesMemorias do Instituto Oswaldo Cruz, 1678-8060, Vol, 97, Nro. 8, 2002, p. 1165-1168spa
dc.relation.urihttps://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000800018&lng=en&tlng=en
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.accessrightshttps://purl.org/coar/access_right/c_abf125
dc.rights.creativecommons2002
dc.rights.localAcceso abiertospa
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/*
dc.subject.decsEnsayo de inmunoadsorción enzimáticaspa
dc.subject.decsFormación de anticuerposspa
dc.subject.decsPruebas inmunológicasspa
dc.subject.keywordsGiardiaspa
dc.subject.keywordsFecesspa
dc.subject.keywordsAntigenspa
dc.titleDetection of giardia duodenalis antigen in human fecal eluates by enzyme-linked immunosorbent assay using polyclonal antibodiesspa
dc.typearticlespa
dc.type.hasversioninfo:eu-repo/semantics/publishedVersion
dc.type.localartículospa

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